MiPNet17.04 CitrateSynthase

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MiPNet17.04 CitrateSynthase

Publications in the MiPMap
Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.

» Bioblast pdf »Versions

Oroboros (2020-04-18) Mitochondr Physiol Network

Abstract: Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12.

Version 04: New Section 6. Normalization of respiratory flux for CS activity Keywords: Normalization of rate

O2k-Network Lab: AT Innsbruck Oroboros

O2k-technical support and open innovation
Open the pdf document above.
» Current O2k-series: O2k-FluoRespirometer
» Current software version: DatLab 7.4

Author contributions

This MiPNet Protocol was originally prepared by Kuznetsov AV, Lassing B, Gnaiger E as MiPNet08.14 (2003) and further edited by Wiethüchter A (2011). MiPNet17.04 (2012 version) was edited by Eigentler A, Draxl A, Gnaiger E; contribution to K-Regio project MitoCom Tyrol, funded by the Tyrolian Government and the European Regional Development Fund (ERDF).
Last updates: contribution to H2020 Project NextGen-O2k.


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Labels: MiParea: Respiration, Instruments;methods 

Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, Enzyme  Enzyme: Marker enzyme 

HRR: Oxygraph-2k, O2k-Protocol 

Mt- and marker-enzymes, Normalization of rate