From Bioblast
MiPNet18.13 IOC84 Alaska
Big Lake, Alaska US, 2014 Jan 04-07. Oroboros O2k-Workshop on HRR and Racing dogs at Iditarod, IOC84. ยปUS OK Stillwater Davis MS |
OROBOROS (2014-01-04) Mitochondr Physiol Network
Abstract:
84th Oroboros O2k-Workshop on high-resolution respirometry in cooperation with O2k-Network Lab US OK Stillwater Davis MS.
Project: Racing dogs at Iditarod, Alaska 2014 with integrated O2k-Workshop
โข O2k-Network Lab: US OK Stillwater Davis MS, AT Innsbruck OROBOROS
IOC84: Racing dogs at Iditarod, Alaska 2014
- A special expertise of the Oroboros mitoscience-team is the logistic planning and support of expeditions with a focus on mitochondrial physiology. IOC84 was integrated in the Alasca expedition as a training course.
- >> IOC84
- Collaboration of Michael Davis and Katherine Williamson (Comparative Exercise Physiology Laboratory, Oklahoma State University) with Martin Buser (Happy Trails Kennel, Alaska), Robert Boushel (Swedish School of Sport and Health Sciences, Stockholm, SE), Benjamin F. Miller and Karyn Hamilton (Colorado State University, US), and Erich Gnaiger and Verena Laner (Medical University of Innsbruck and Oroboros Instruments) - a Power-O2k project with six Oroboros O2k.
Results published
- Data generated in this collaborative study are published: Miller 2017 PLOS ONE.
Lecturers and tutors
- Davis Michael S, Professor, Physiological Sciences Oxley Equine Professor
- Director, Comparative Exercise Physiology Lab
- Gnaiger Erich, CEO, Oroboros Instruments
- Laner Verena; Project manager and application specialist, Oroboros INSTRUMENTS
- Boushel RC; Professor and Chair in Physiology, The Swedish School of Sport and Health Sciences
- Alaska Expedition pre-study ("Iditarod"). Collaboration with Michael Davis (Oklahoma State University, US), Robert Boushel (Swedish School of Sport and Health Sciences, Stockholm, SE) and Benjamin F. Miller (Colorado State University, US) - with integrated IOC84 (Jan 04-07).
- Dr. Erich Gnaiger (Medical University of Innsbruck and Oroboros Instruments) and his collaborator Verena Laner (Oroboros Instruments) joined the project of Michael Davis (Oklahoma State University) in Big Lake, Alaska. Cumulative training effects on the mitochondrial function of alaskan sled dogs were studied in January and March 2014 at Happy Trails Kennel in Big Lake, Alaska. Further participants were Robert Boushel (Institute for Sport and Health Sciences, Stockholm, SE) as well as Benjamin F. Miller and Karyn Hamilton (Colorado State University, Fort Collins, US).
Racing dogs at Iditarod, Alaska 2014
- Iditarod - The last great race(r) 2014: the greatest challenge up to the finish: ยป iditarod.com
- At Martin Buser's 'Happy Trails Kennel' (Big Lake, near Anchorage, Alaska) Prof. Michael Davis (Comparative Exercise Physiology Laboratory, Oklahoma State University, USA) conducts a long-term collaboration with Martin's team to study and train the Alaskan dogs in preparation of long-distance races, the most challenging of which is the 1,100 mile Iditarod race. Martin Buser is 4 times champion of the Iditarod, with a scientifically oriented interest in the study of the genetics and physiology of the dogs that goes far beyond the race.
- The line of mixed-breed Alaskan huskies of Martin incorporates sprint-dog lines and is selected and trained as much for long-distance endurance as for speed - and happiness. "Happy dogs perform better and make racing more fun" (Martin Buser).
- ยป buserdog.com
- In January and March 2014, Mike Davis and Katherine Williamson teamed up with Erich Gnaiger and Verena Laner (Medical University of Innsbruck and Oroboros Instruments), Robert Boushel (Swedish School of Sport and Health Sciences, Stockholm, SE), and Benjamin F. Miller and Karyn Hamilton (Colorado State University, US) to focus on mitochondrial respiratory function in the skeletal muscle of the dogs. With a Power-O2k approach using six Oroboros O2k, these mitochondrial studies are conducted at the beginning of the intensive training period and after the Iditarod race, considered as the final stage of the most intensive endurance training.
The tutors
The science team
Impressions of the study
The fast endurance heroes
The mitochondrial lab at Happy Trails Kennel
- Dog skeletal muscle mitochondria: the first detailed study with SUIT protocols.
Getting connected
What do the dogs tell us?
A visitor at Happy Trails Kennel
The mito team
Mike Davis and Erich Gnaiger discuss the SUIT protocols.
Alaska Expedition main study (March 2014)
- Trained cohort
- SUIT protocols SUIT_FNS(PGM)03, SUIT_NS(PGM)04
Titrations for SUIT protocols
Permeabilized fibre preparation
- Fotos by Gnaiger Erich and Laner Verena
Further links of interest
- Youtube
- k300: Congratulations to Rohn Buser for winning this race. "Rohn won it in 38 h and change, but not without a MAJOR challenge from Jeff King who made a run at Rohn in the last 5-10 miles, but couldn't overtake him. 300 miles (500 km) in 38 h. When you subtract the 10 h of mandatory rest, that means Rohn's dogs AVERAGED nearly 18 kph for over a day. These are the dogs we will investigate in March. That isn't even the race record. Ironically, the race record of 37 h 4 min is held by Martin Buser" (Michael S. Davis, 2014-01-20).
Recommended reading
- Miller B, Hamilton K, Boushel R, Williamson K, Laner V, Gnaiger E, Davis M (2017) Mitochondrial respiration in highly aerobic canines in the non-raced state and after a 1600-km sled dog race. PLOS ONE 12:e0174874. - ยปBioblast linkยซ
- O2k-Core Manual
- SUIT protocols for high-resolution respirometry
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. ยปBioblast Access
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. ยปBioblast Access
- SUIT protocols for high-resolution respirometry
- Mitochondrial pathways
- Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp. - ยปBioblast linkยซ handout to O2k-Workshop participants.
- Mitochondrial pathways
- O2k-Workshops are listed as MitoGlobal Events.
- This workshop is a component of the K-Regio project MitoCom.
- O2k-Workshops are listed as MitoGlobal Events.
Labels:
HRR: Oxygraph-2k
ORO, IOC, 2014, O2k-Network, MitoCom