MiPNet27.08 Innsbruck BEC tutorial-Living Communications pmF

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Bioenergetics Communications        
Gnaiger 2020 BEC MitoPathways
Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1.
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Innsbruck AT, 2022 Sep 19. BEC tutorial-Living Communications: pmF

MiPsociety (2022-09-19) Mitochondr Physiol Network

Abstract: BEC tutorial-Living Communications. Mitochondrial membrane potential and Peter Mitchell’s protonmotive force: elements of the science of bioenergetics.

Gnaiger 2020 BEC MitoPathways

The mitochondrial membrane potential is an element of the science of bioenergetics, linked to the control of respiratory flux and related mitochondrial functions. A PubMed search on ‘mitochondrial membrane potential’ yields nearly 40 000 results and 3442 for 2021 (search 2022-07-04), with a linear increase during the past 20 years. Chapter 8 on ‘Protonmotive pressure and respiratory control’ of Mitochondrial Pathways (Gnaiger 2020) introduces a novel perspective on Peter Mitchell’s protonmotive force, which incorporates the mitochondrial membrane potential. If you find the reading is tough, you are not alone. Join this BEC tutorial-Living Communications for an introduction into the relevant concepts of physical chemistry, which differ from misleading chapters in bioenergetics textbooks on potential gradients, Gibbs energy, protonmotive flow and force, and finally protonmotive pressure. This will introduce students (and teachers) to a new understanding of mitochondrial membrane potential and the protonmotive force, connecting the ideal gas equation, osmotic pressure, the Boltzmann constant and gas constant with Fick’s and Einstein’s diffusion equation. If theory gets tough, join for a follow-up retreat.

O2k-Network Lab: AT_Innsbruck_Oroboros

Figure 1.1. Coupling in oxidative phosphorylation is mediated by the protonmotive force pmF.

Last update: 2022-09-18


MiPArt at Oroboros Instruments
Schöpfstr 18
6020 Innsbruck


A discussion-oriented presentation
Erich Gnaiger (AT) presents the concept of protonmotive pressure in discussion with Jean-Pierre Mazat (FR) and participants.
  • Blocks of 10 + 5 min comments, questions, discussion
  • Sections of Chapter 8, Blue Book
Monday, Sep 19Protonmotive force pmF = ΔmFH+ and protonmotive pressure ΔmΠH+
Time Section Topic a b c d
13:00-13:10 8.1.-8.3. Overview pmF equation Mitchell's four modules three introductory topics
13:15-13:25 8.4. 1. Four protonmotive theorems diffusive pressure gradient pressure difference and potential difference diffusive and electric pressure difference mt-volume fraction: concave to convex flow/force
13:30-13:40 8.1. 2. Vectorial and scalar quantities amount and charge format diffusion: gradients and compartments stoichiometry and advancement
13:45-13:55 8.2. 3. Why is thermodynamics scary? from thermodynamics to ergodynamics chemical potential metabolic force Gibbs energy - exergy
14:00-14:10 8.2.-8.3. 4. Protonmotive force and motive units elementary quantities and motive units pmF formats conversion between formats measurement of the pmF
14:15-14:45 Coffee/tea - Chat
14:45-14:55 8.4.1. 5. Protonmotive pressure pmP linearity diffusion gradients and Einstein's diffusion equation Fick's law pressure-force confusion thermodynamics of irreversible processes
15:00-15:10 8.4.2. 6. Compartments: diffusion and osmotic pressure concave flux/force relation free activity infinite forces without explosion
15:15-15:25 8.4.3. 7. Hydrogen ions and counterions if the force is in ∆pH electroneutral exchange of counterions equilibrium H+ and counterion distribution concave flow (pressure)/force relation
15:30-15:45 8.4.4. 8. Matrix volume fraction and flux-pressure linearity anodic volume fraction closed to open anodic system from intensity to capacity non-ohmic proton leak explained by first principles
15:45-16:00 General discussion with a glass of wine - a taste of Gentle Science
» Programme: BEC tutorial-Living Communications: pmF to pmP


Recommended reading

Hydrogen ion circuit and coupling in OXPHOS
Gnaiger 2020 BEC MitoPathways
  1. Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. https://doi.org/10.1016/j.bbabio.2011.09.018
  2. Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002 - Chapter 8
  3. Gnaiger E (2021) The elementary unit — canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. https://doi.org/10.26124/mitofit:200004.v2

» MitoPedia: Ergodynamics

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