MiPschool 2007 Schroecken AT Programme

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MiPschool 2007 Schroecken AT Programme

MiPsummer Programme

12-18 July 2007, Schroecken, Vorarlberg, Austria
Lectures of the MiPsummer School on "Mitochondrial Respiratory Phyisology" will provide a balance between an introduction into the basic concepts, advanced methodological and scientific approaches, and specific applications, with a focus on mitochondrial respiratory function linked to the general theme of MiP.
For addresses of lecturers, see MiPsummer Participants.
Day 1: Mitochondrial respiratory capacity, coupling and respiratory control. Programme 1 - Abstracts 1
Day 2: Basic science and clinical reality 1. Programme 2 - Abstracts 2
Day 3: Oxygen kinetics of mitochondrial respiration and inhibition by nitric oxide. Programme 3 - Abstracts 3
Day 4: Mitochondrial membrane potential. Programme 4 - Abstracts 4
Day 5: Basic science and clinical reality 2. Programme 5 - Abstracts 5

1 - Friday, 13. July

MiPsummer Morning Lectures. Mitochondrial respiratory capacity, coupling and respiratory control - Abstracts Day 1
Chair: Steven Hand (US), Susana Cadenas (ES)
1L-01 09:00-10:00 Erich Gnaiger (Innsbruck, AT) MitoPathways and mitochondrial respiration in top gear – an introduction to the concept of the ‘Q-junction’ and respiratory control with multiple substrates.

Reading: Gnaiger E (2007) in: Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck: pp.21-37.

1L-02 10:00-11:00 Erich Gnaiger (Innsbruck, AT) P/O, respiratory and uncoupling control ratios – established concepts re-visited.

Reading: Gnaiger E (2001) Respir. Physiol. 128: 277-297.

11:00 Coffee
1L-03 11:45-12:45 Kathrin Renner (Regensburg, DE) Development of a respiratory protocol to detect mitochondrial defects under drug treatment.

Reading: Renner et al (2003) Biochim. Biophys. Acta 1642: 115-123.

13:00 Lunch
MiPsummer Afternoon Workshop: Permeabilized muscle fibers and high-resolution respirometry
1W 15:00-19:00 Hélène Lemieux, Erich Gnaiger (Innsbruck, AT)

Permeabilized fiber preparation: Mouse heart. Multiple substrate/inhibitor protocol: A lecture hall experiment on-line. Cytochrome c oxidase inhibitor titration – biochemical threshold and excess capacity.

17:00-18:15 Coffee and Posters
1P-01 Marco Alves, Paulo J Oliveira, Rui A Carvalho (PT) Preservation of cardiac mitochondrial function during ischemia and ischemia-reperfusion: role of the cardioplegic protection by Celsior and Histidine buffer solution.
1P-02 Dominique-Marie Votion (BE), Hélène Lemieux, Ange Mouithys-Mickalad, Didier Serteyn, Erich Gnaiger. Study of the mitochondrial respiration in the equine muscle with high-resolution respirometry: feasibility, preliminary results and potential applications.
1L-04 18:15-19:00 Hélène Lemieux (Innsbruck, AT) High excess capacity of cytochrome c oxidase in permeabilized fibers of the mouse heart.

Reading: Lemieux H, Gnaiger E (2007) in: Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck: pp. 38-42.

20:00 Dinner at Hotel Mohnenfluh'

2 - Saturday, 14. July

MiPsummer Morning Lectures. Basic science and clinical reality
Chair: Kathrin Renner (DE), Erich Gnaiger (AT)
2L-01 09:00-10:00 Hong Kyu Lee (Seoul, KR) The metabolic syndrome and mitochondrial function - towards an international mtDNA cybrid study group.
2L-02 10:00-11:00 Jaap Keijer (Wageningen, NL) Bioactive food components and energy metabolism.
11:00 Coffee
2L-03 11:45-12:45 Vilma Borutaite (Kaunas, LT) Mitochondrial regulation of cell death: NO and cytochrome c redox state.

Reading: Mander P et al.(2005) J. Neurosci. Res. 79: 208-215.

13:00 Lunch
MiPsummer Special Topic: Basic science and clinical reality
2L-04 15:00-15:45 Hong Kyu Lee (Seoul, KR) Diabetes 2 and the role of mitochondrial pathophysiology.

Flash presentations of posters:

2P-01 15:50-16:00 Sandra Amaral, Luís Martins, Paula C. Mota, Paulo J. Oliveira, João Ramalho-Santos (PT) Mitochondrial testicular function: Effects of age and diabetes
2P-02 16:00-16:10 Timothy R Koves, Noland RC, Slentz DS, Ilkayeva O, Newgard CB, Muoio DM (US) Mitochondrial dysfunction and metabolic inflexibility in models of aging and diet-induced obesity.
2P-03 16:10-16:20 Joris Hoeks, JJ Briedé, J de Vogel, G Schaart, M Nabben, E Moonen-Kornips, MKC Hesselink, P Schrauwen (NL) Mitochondrial function, -content and -ROS production in rat skeletal muscle: effect of high-fat feeding.
2P-04 16:20-16:30 Maxi Meissner, G Henk Visser, Frans Stellaard, F Kuipers (NL) Bile acid signaling, FXR and energy expenditure – novel perspectives for the treatment of metabolic diseases.
16:30–19:00 Coffee and Posters
2P-05 Ruwen Böhm, L Schild, Th Herdegen (DE) Different approaches to assessing mitochondrial function and their utility and accuracy for pharmaceutical testing.
2P-06 Peter Lindinger, Martine Calame, Matthias Hoch, Alex N Eberle, Ralph Peterli, Thomas Peters, Sjouke Hoving, Hans Voshol, Ian Fearnley, John E Walker (CH) Proteome analysis of mitochondria in adipose tissues of Bariatric patients: Identification of risk factors and/or predictors for the treatment outcome.
2P-07 Alena Čížková, Stránecký V, Ivánek R, Hartmannová H, Nosková L, Piherová L, Tesařová M, Hansíková H, Honzík T, Zeman J, Divina P, Paul J, Houštěk J, Kmoch S (CZ) h-MitoArray and gene expression analysis in 13 patients with mitochondrial ATP synthase deficiency.
2P-08 Daniela Fornuskova, Stiburek L, Pejznochova M, Zeman J (CZ) Depletion of COX5A subunit in HEK293 cells.
2P-09 Virginie Rhein, G Baysang, F Meier, L Ozmen, H Bluethmann, E Savaskan, F Müller-Spahn, C Czech, J Götz, A Eckert (CH) Changes in mitochondrial respiration in brain cells of transgenic mice models of Alzheimer’s disease
2P-10 Olga Brantova, J Sladkova, H Hansikova, J Zeman (CZ) Fibroblasts cell culture as model for apoptosis detection.
2P-11 Karin Eberhart, Ireen Ritter, Birgit Timischl, Reinhard Kofler, Peter Oefner, Kathrin Renner (DE) Synergistic effect of glucocorticoids and 2-deoxyglucose - energy metabolism as a new target in the treatment of leukemia.
2P-12 Zdeněk Paris, Julius Lukeš (CZ) Functional analysis of genes encoding mitochondrial proteins in Trypanosoma brucei.
20:00 Dinner at Hotel Mohnenfluh

3 - Sunday, 15. July

MiPsummer Morning Lectures: Oxygen kinetics of mitochondrial respiration and inhibition by nitric oxide
Chair: Vilma Borutaite (LT), Jaap Keijer (NL)
3L-01 09:00-10:00 Erich Gnaiger (Innsbruck, AT) Respiratory control by oxygen pressure under normoxia and hypoxia.

Reading: Gnaiger E (2003) Adv. Exp. Med. Biol. 543: 39-55.

3L-02 10:00-11:00 Alessandro Giuffre (Rome, IT) Inhibition of respiratory oxidases by NO: Mechanistic and physiological aspects.

Reading: Brunori M et al (2006) Biochim. Biophys. Acta. 1757:1144-1154.

11:00 Coffee
3L-03 11:45-12:45 Susana Cadenas (Madrid, ES) Inhibition of cellular respiration by edogenous nitric oxide and relative sensitivity of the main two cellular targets of nitric oxide.

Reading: Rodríguez-Juárez et al (2007) Biochem. J. 405: 223–231.

13:00 Lunch
MiPsummer Afternoon Workshop: Spectrophotometry and oxygen kinetics. – NO and kinetic models.
3W 15:00-17:00 Natascha Sommer(DE), Francesca Scandurra, Thomas Derfuss, Erich Gnaiger. Simultaneous remission spectrophotometry and high-resolution respirometry. Respiratory control by oxygen and redox states of mitochondrial cytochromes in cells: Instrument demonstration.
17:00-18:00 Coffee and Posters
3P-01 Enara Aguirre, F Rodríguez-Juárez, E Gnaiger, S Cadenas (ES) Inhibition of cellular respiration by nitric oxide during normoxia and hypoxia.
3P-02 Felix Rodríguez-Juárez, E Aguirre, S Cadenas (ES) Relative sensitivity of soluble guanylate cyclase and mitochondrial respiration to endogenous nitric oxide at physiological oxygen concentration.
3D 18:00-18:30 Discussion: Oxygen kinetics, NO and models of competitive and non-competitive inhibition.
19:00 Dinner at Hotel Mohnenfluh
20:15 Open air folk music – garden of Hotel Mohnenfluh.

4 - Monday, 16. July

MiPsummer Morning Lectures: Mitochondrial membrane potential and respiratory control.
Chair: Rodrigue Rossignol (FR), Alessandro Giuffre (IT)
4L-01 09:00-10:00 Vilma Borutaite (Kaunas, LT) Mitochondrial membrane potential and respiratory control.
4L-02 10:00-11:00 Steven C Hand (Baton Rouge, US) Mitochondrial membrane potential, pH gradients, and redox state.

Reading: Menze MA et al (2005) Am. J. Physiol. Regul. Integr. Comp. Physiol. 289: R68–R76.

11:00 Coffee and Posters
4P-01 Magdalena Labieniec, Cezary Watala, Dominika Malinska (PL) Resorcylidene aminoguanidine (RAG) affects calcium mobilization and transmembrane potential in isolated rat liver mitochondria without impairments of respiratory chain.

Flash presentations and posters:

4P-02 11:30-11:40 Dominika Malińska, Adam Szewczyk (PL) Mitochondrial potassium channel opener BMS-191095l – cytoprotective properties and the influence on mitochondrial physiology.
4P-03 11:40-11:50 David Julian, Allman Rollins (US) Mitochondrial movement is by Brownian motion in coelomocytes from an annelid worm.

In case of favourable weather conditions:

12:00 MiPsummer Walk to the cheese and wine reception at the Alpmuseum uf m Tannberg; refreshment in the lake Körebersee or at Hotel Körbersee.'
18:30 MiPsummer Grill Party.
21:00 Meeting of the MiPboard – MiP2008 (round table conference), MiP2009 (joint conference with UMDF), future perspectives (open for all participants).

5 - Tuesday, 17. July

MiPsummer Morning Lectures: Basic science and clinical reality
Chair: Hong Kyu Lee (KR), Dominique-Marie Votion (BE)
5L-01 09:00-10:00 Rodrigue Rossignol (Bordeaux, FR) Mitochondrial bioenergetics and structural network organization.
5L-02 10:00-11:00 Enrico Calzia(Ulm, DE) Small and large animal models of sepsis and I/R-injury - how to combine basic science and clinical reality.
11:00 Coffee
5L-03 11:45-12:45 Charles Hoppel (Cleveland, US) Integrated mitochondrial function and the clinical utility of polarographic analysis.
13:00 Lunch
15:00 MiPsummer Afternoon Workshop: Lectures on open topics; Round table discussion; Summary - evaluation
20:00 MiPsummer Dinner at Hotel Mohnenfluh
5A 22:00 Charles Hoppel (Cleveland, US) Inner mitochondrial membrane and outer space.