O2k-Catalogue: O2k-Modules
| Description | 4#### O2k-Catalogue: O2k-Modules. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user. |
|---|---|
| Product ID | 4#### |
| Type | O2k, MultiSensor |
| Link | |
| Image | 4#### |
O2k-Modules
O2k-Modules are add-on modules supported by the O2k. When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an O2k-MultiSensor application. The O2k fully supports the O2k-Modules.
- MiPNet17.05 O2k-Fluo LED2-Module
- O2k-TPP+_ISE-Module
- O2k-pH ISE-Module
- O2k-NO Amp-Module
- Hydrogen peroxide
- Cytochrome Spectra and Oxygen
High-resolution respirometry is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H2O2 production, mitochondrial membrane potential, intracellular pH, Ca2+, Mg2+, and NADH levels.
Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
- Quality control of preparations by respirometric performance.
- Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP+ or fluorophores.
- Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
- Additional information is acquired for a limited amount of sample.
- Oxygen dependence of parameters can be studied.
O2k-Catalogue: O2k-Modules
O2k-MultiSensor Manual
| Chapter | Section | Last update |
|---|---|---|
| MiPNet15.03 O2k-MultiSensor-ISE |  | 2021-08-02 |
| MiPNet15.05 NO-manual | | 2016-04-21 |
| MiPNet17.05 O2k-Fluo LED2-Module | | 2021-06-22 |
| MiPNet23.15 O2k-pH ISE-Module | | 2018-09-08 |
| MiPNet24.12 NextGen-O2k: Q-Module | | 2021-10-29 |
| MiPNet26.11 NextGen-O2k: PB-Module | | 2021-10-29 |
| MiPNet26.12 NextGen-O2k: NADH-Module | | 2022-05-17 |
| MiPNet26.13 NextGen-O2k manual | | 2022-07-25 |
References
- Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - »Bioblast link«
- Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - »Bioblast link«
