Oemer 2021 J Lipid Res

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Oemer G, Edenhofer ML, Wohlfarter Y, Lackner K, Leman G, Koch J, Cardoso LHD, Lindner HH, Gnaiger E, Dubrac S, Zschocke J, Keller MA (2021) Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells. J Lipid Res 100111. DOI:https://doi.org/10.1016/j.jlr.2021.100111.

» JLR Open Access

Oemer Gregor, Edenhofer Marie-Luise, Wohlfarter Yvonne, Lackner Katharina, Leman Geraldine, Koch Jakob, Cardoso Luiza HD, Lindner Herbert H, Gnaiger Erich, Dubrac Sandrine, Zschocke Johannes, Keller Markus A (2021) J Lipid Res

Abstract: The molecular assembly of cells depends not only on the balance between anabolism and catabolism, but to a large degree on the building blocks available in the environment. For cultured mammalian cells, this is largely determined by the composition of the applied growth medium. Here we study the impact of lipids in the medium on mitochondrial membrane architecture and function by combining LC-MS/MS lipidomics and functional tests with lipid supplementation experiments in an otherwise serum- and lipid-free cell culture model. We demonstrate that the composition of mitochondrial cardiolipins strongly depends on the lipid environment in cultured cells and favours the incorporation of essential linoleic acid over other fatty acids. Simultaneously, the mitochondrial respiratory Complex I activity was altered, whereas the matrix-localized enzyme citrate synthase was unaffected. This raises the question on a link between membrane composition and respiratory control. In summary, we found a strong dependency of central mitochondrial features on the type of lipids contained in the growth medium. This underlines the importance of considering these factors when using and establishing cell culture models in biomedical research.

Bioblast editor: Gnaiger E O2k-Network Lab: AT Innsbruck Oroboros

Labels: MiParea: Respiration, mt-Membrane 

Organism: Human  Tissue;cell: HeLa  Preparation: Permeabilized cells  Enzyme: Complex I, Marker enzyme  Regulation: Cyt c, Flux control, Fatty acid  Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k 

SUIT-008, TissueModels