|Title||Product description||Product ID||Image|
|O2k-FluoRespirometer||The Oroboros O2k-FluoRespirometer (O2k-Series H) - the experimental system complete for high-resolution respirometry (HRR), including fluorometry and the TIP2k, allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+ (CaG) or ATP production (MgG).
The O2k-FluoRespirometer supports all add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module, enabling measurement of mt-membrane potential with ion sensitive electrodes (ISE for TPP+ or TPMP+ ) or pH.
- oxygen consumption
- ROS production
- ATP production
- mitochondrial membrane potential
Features of O2k-Series H:
- Fluo-Control Unit integrated into O2k-Main Unit.
- Smart Fluo-Sensors with pre-calibrated light intensities for direct input into DatLab 7
- Added: spare OroboPOS
- DL-Protocols as real-time guides for titrations in DatLab 7, context-sensitive help. Further details » MitoPedia: DatLab.
The O2k is a sole source apparatus with no other instruments meeting its specifications.
|Power O2k-FluoRespirometer||Power O2k-FluoRespirometer - optional configuration as additional system for increasing output combined with the O2k-FluoRespirometer (O2k-Series H). The Power O2k-FluoRespirometer includes the TIP2k, and supports all add-on O2k-Modules of the Oroboros O2k. It can be added to an existing Oroboros O2k of any O2k-Series. This application does not require an additional ISS-Integrated Suction System and O2k-Titration Set. Furthermore, the following OroboPOS Service Tools can be used from the available O2k and are not included: OroboPOS-Mounting Tool.||10003-02|
|Startup O2k-Respirometer||Startup O2k-Respirometer - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Respirometer includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Respirometer supports all add-on O2k-Modules of the Oroboros O2k, and is a component of the O2k-FluoRespirometer.
On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software.
The O2k is a sole source apparatus with no other instruments meeting its specifications.
The Power O2k-Respirometer is an economical option for using additional O2k-Units to increase output in high-resolution respirometry. This application does not require an additional ISS-Integrated Suction System, an O2k-Titration Set and the O2k-Manual. Furthermore, the following OroboPOS Service Tools can be used from the available O2k and are not included: OroboPOS-Mounting Tool.
The Power O2k-Respirometer supports all add-on O2k-Modules of the Oroboros O2k.
|TIP2k-Module||TIP2k-Module - Titration-Injection microPump (TIP2k) for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat). The TIP2k-Module includes TIP2k-Accessories: 4 Microinjection syringes, 1 Pkg. TIP2k-Needle Spacers with mounting tool, 1 TIP2k-Needle Safety Support and 1 Pkg. TIP2k Filter Papers, and the newest DatLab version.||11100-03|
|O2k-sV-Module||O2k-sV-Module: 12 mm inner diameter, Duran glass polished, with smaller operation volume (V) of 0.5 cm3 (0.5 mL), to perform high-resolution respirometry with reduced amount of biological sample.||11200-01|
|O2k-TPP+ ISE-Module||O2k-TPP+ ISE-Module: 2 potentiometric ion-selective electrodes (ISE), ISE-Service Kit and 2 PEEK-Stoppers, supported by the Oroboros O2k (O2k-Series D and higher). It also includes the newest DatLab Version. The ISE module is designed with replaceable membranes making it possible to measure different ions such as TPP+ or Ca2+ with the same electrode housing. The main application of the O2k-TPP+ ISE-Module is measurement of mitochondrial membrane potential with tetraphenylphosphonium (TPP+) or TPMP+.||12300-03|
|O2k-pH ISE-Module||O2k-pH ISE-Module: two pH electrodes and reference electrodes and accessories, two black PEEK stoppers, supported by the Oroboros O2k. It also includes the newest DatLab version.
Quality control: Stability of Oroboros pH electrodes is tested for drift <15 µpH·s-1·mL-1.||12400-02|
|O2k-NO Amp-Module||O2k-NO Amp-Module: 2 black PEEK stoppers for amperometric O2k-MultiSensor application (NO, H2S, H2O2); 1 additional hole (2.3 mm); central titration port; conical bottom; with Volume-Calibration Rings (A and B); 2 O-rings mounted on each stopper, with 16 spare O-rings (O-ring\Viton\12.5x1 mm; two boxes). The NO sensor is not included.||12500-02|
|PBI-Shredder O2k-Set||PBI-Shredder O2k-Set: Auxiliary O2k-Tool for tissue homogenate preparation; the Shredder-Kit Box contains the heavy duty high torque SG3 driver with convertible handle, SG3 base with 3 position force setting lever (FSL), battery charger, two lithium ion batteries, Shredder-Tube Cap Tool and Shredder-Tube Ram Tool. The PBI-Shredder O2k-Set includes the Shredder-Kit Box with 100 Shredder-Tubes FT500-PS, 100 Shredder-Tubes FT500-PMS, a pair of sharp forceps for tissue dissection, a pair of scissors and a manual.
Oroboros Instruments: world-wide distributor.||13200-04|
|Microbalance-Set||Microbalance-Set: Microbalance 120 g (41 g to 120 g; 0.01 mg / 0.1 mg display; 100 to 240 V) without Microbalance-Transport Case; particularly for wet weight determination of permeabilized fibres.
Microbalance-Transport Case is not suitable for shipping, but is available upon request.||13310-01|
|O2k-Dissection Set||O2k-Dissection Set: for tissue preparation, set of 4 pairs of stainless steel, antimagnetic forceps and a pair of scissors.||13400-01|
|DatLab||DatLab is the O2k-Software for Data Acquisition & Analysis, specifically developed for high-resolution respirometry with the O2k-FluoRespirometer.
The newest DatLab version is DatLab 7.4, included in the O2k-FluoRespirometer. Updates of DatLab are free of charge during the warranty period of the O2k.
The minimum computer requirements are Intel-Core-2 or equivalent CPU, 2GB RAM and Windows XP. However, we recommend Intel i5 or equivalent CPU, 4GB RAM, Windows 10 and SSD. For the proper display of DatLab on your computer, please make sure the “Language settings” are set to English.
|ISS-Integrated Suction System||ISS-Integrated Suction System: Suction pump 230 or 120 V with stainless steel housing, 2 liter waste bottle, filter and tubing; for siphoning off excess medium from the O2k-Stopper and for emptying the O2k-Chambers. The ISS is included as a standard component of the O2k-FluoRespirometer. Media containing living cells or microorganisms, various poisons (inhibitors, uncouplers) and mixtures of proteins and substrates are safely disposed off in the 2-litre waste bottle.
The suction pump (230 V or 120 V) is enclosed in a stainless steel housing, which holds a readily accessible mains switch, two removable receptacles for the tip of the tubing, an easily removable and safe connection to the gas filter which further connects to the fully stabilized waste bottle. The waste bottle with filter can be simply removed for emptying, cleaning and drying.
For specific country configurations, see Product ID 28101, 28102, 28103.
|O2k-Titration Set||The O2k-Titration Set consists of Hamilton microsyringes (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 1 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3; fixed needles with rounded tips), provided in the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks.
The O2k-Titration Set is included in the O2k-FluoRespirometer and Startup O2k-Respirometer.||20820-03|
|O2k-Catalogue: O2k-Assembly Kit||21### O2k-Assembly Kit: The O2k-Assembly Kit is a component of the O2k-FluoRespirometer, consisting of 2 PVDF Stirrer-Bars, 2 PVDF or PEEK O2k-Stoppers, OroboPOS-Connectors (attached to the O2k-Main Unit) and cables (power supply, USB-connection). Several components of the O2k-Catalogue: O2k-Assembly Kit are included in the O2k-Service Box either for shipment or for storage.||21###||21###|
|OroboPOS-Connector||OroboPOS-Connector (blue POM), with male connection to OroboPOS sensor head (POS) and with cable and male plug fitting into O2k-Main Unit.
Two units of this item are standard components of the O2k-Assembly Kit (O2k-FluoRespirometer), mounted on the OroboPOS-Holders at the O2k-Main Unit.||22200-01|
|O2k-Chamber||O2k-Chamber: 16 mm inner diameter, Duran glass polished, with standard operation volume (V) of 2 cm3 (2 mL).
Two units of this item are standard components of the O2k-FluoRespirometer. Separate glass chambers are available as spares. The optical properties of Duran allow application of fluorometric sensors (Duran optical properties).||23100-01|
|Stirrer-Bar\white PVDF\15x6 mm||Stirrer-Bar\white PVDF\15x6 mm, operated in the O2k-Chamber at constant stirring speed (standard is 750 rpm, or 12.5 Hz), to provide optimum mixing of the sample in the aqueous medium and ensure a stable signal of the polarographic oxygen sensor (OroboPOS) placed in a position of maximum current of the medium.
O2k-FluoRespirometer: 2 in O2k-Assembly Kit.
Two units of this item are standard components of the O2k-Assembly Kit (O2k-FluoRespirometer).
|Stirrer-Bar\black PEEK\15x6 mm||Stirrer-Bar\black PEEK\15x6 mm, for specific applications with light guides to minimize optical interference.||23220-01|
|Stopper\black PEEK\conical Shaft\central Port||Stopper\black PEEK\conical Shaft\central Port: with conical shaft and one central capillary (with PTFE, graphite, carbon fiber), Volume-Calibration Ring (A or B) for volume adjustment 1.5 to 3.2 ml; 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm). The black PEEK stoppers are required for optical O2k-MultiSensor Modules (O2k-Fluo_LED2-Module).
Two units of this item are standard components of the O2k-Assembly Kit.||24000-01|
|O-ring\Viton\12.5x1 mm||O-ring\Viton\12.5x1 mm, for PVDF or PEEK O2k-Stoppers, box of 8 as spares.
Two spare boxes of this product are standard components of the O2k-Assembly Kit (O2k-FluoRespirometer) as well as the O2k-TPP+ ISE-Module, O2k-pH ISE-Module and the O2k-NO Amp-Module.||24310-02|
|Volume-Calibration Ring||Volume-Calibration Ring (A or B) PVDF for O2k-Stoppers, white; attached to the stoppers.||2432A-01 or 2432B-01|
|Cover-Slip\black||A Cover-Slip should be placed on top of the O2k-Stopper to minimize contamination and evaporation of liquid extruding from the capillary of the stopper. The Cover-Slips do not exert any direct effect on oxygen backdiffusion into the O2k-Chamber. Use the the Cover-Slip\black to avoid light penetration and disturbance of fluorescence signals and generally for optical measurements in the O2k.
Two units of this item are standard components of the O2k-Assembly Kit.||24411-01|
|Stopper-Spacer||Stopper-Spacer (or gas spacer) to set the O2k-Stopper into a standard position for a fixed gas phase above the aqueous phase in the O2k-Chamber, during air calibration or for injection of nitrogen, argon or oxygen into the gas phase; Stopper-Spacer thickness of 4mm (a deviation of +/- 10% possible).
One Stopper-Spacer is included in the O2k-Assembly Kit (O2k-FluoRespirometer), two in the O2k-TPP+ ISE-Module as standard components.||24420-01|
|Syringe\60 ml\Gas-Injection||Syringe\60 mL\Gas-Injection, 60 mL, with spacer and stainless steel needle, flat tip, for gas injection into the O2k-Chamber.
This item is a standard component of the O2k-Assembly Kit.||24430-01|
|O2k-Catalogue: OroboPOS components||26### O2k-Catalogue: OroboPOS components||26###||26###|
|OroboPOS||The OroboPOS is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range.
It consists of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 µm membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.||26000-01|
|OroboPOS-Seal Tip||OroboPOS-Seal Tip, black butyl rubber gasket with 3 mm pore. 4/Pck, for sealing the OroboPOS (sensor head) against the O2k-Chamber. Push the wetted OroboPOS-Seal Tip over the POS-sensor head, with the pore positioned centrally, not covering any peripheral area of the gold cathode. Do not stretch the gasket across the POS-sensor head.
One pack (4 seal tips) of this product is a standard component of the OroboPOS-Service Kit.||26110-01|
|OroboPOS-Membranes||OroboPOS-Membranes, FEP 25 µm; 40/Pck.
Two packages of this item are standard components of the OroboPOS-Service Kit.||26200-01|
|OroboPOS-Electrolyte Powder||OroboPOS-Electrolyte Powder, KCl.
This item is a standard component of the OroboPOS-Service Kit.
The powder is dissolved in 10 ml distilled water to yield a 1 M KCl solution.
|OroboPOS-Mounting Tool||OroboPOS-Mounting Tool for application of OroboPOS-Membranes. It consists of two parts, (i) the membrane guide (larger diameter) and (ii) the membrane ring holder with O-ring\Viton\6x1 mm for holding the OroboPOS-Membrane Ring during membrane application.
This item is a standard component of the OroboPOS-Service Kit.||26400-02|
|OroboPOS-Polishing Cloth||OroboPOS-Polishing Cloth for cathode cleaning. Replace the OroboPOS-Polishing Cloth at intervals. A two-year interval may be considered in cases of intensive use.
This item is a standard component of the OroboPOS-Service Kit.||26510-01|
|OroboPOS-Polishing Powder||OroboPOS-Polishing Powder for cathode cleaning.
This item is a standard component of the OroboPOS-Service Kit.||26520-01|
|O2-Zero Powder||O2-Zero Powder, dithionite (Na2S2O4), for zero-oxygen calibration of the OroboPOS.
This item is a standard component of the OroboPOS-Service Kit.||26600-01|
|Forceps for membrane application||Forceps for membrane application: for OroboPOS and ISE membrane application; do not use for tissue preparation.
This item is a standard component of the OroboPOS-Service Kit and the O2k-TPP+ ISE-Module.||26700-01|
|Pipette\Plastic\1 ml ungraded||Pipette\Plastic\1 mL ungraded, for filling electrolyte into the reservoir of the OroboPOS.
This item is a standard component of the OroboPOS-Service Kit.||26800-01|
|ISS-Waste Bottle||ISS-Waste Bottle, 2-liter, component of the ISS-Integrated Suction System.||28120-01|
|ISS-Lid||ISS-Lid, for ISS-Waste Bottle, component of the ISS-Integrated Suction System.||28130-01|
|ISS-Filter and Tubing||ISS-Filter and Tubing, ISS-Integrated Suction System.||28140-01|
|Power O2k Numbers||Power O2k Numbers: Single number to label the O2k in a Power O2k-Lab.||28200-01|
|O2k-Catalogue: TIP2k||3#### O2k-Catalogue: TIP2k||3####||3####|
|Titration-Injection microPump||Titration-Injection microPump (TIP2k) for two-channel operation with the O2k with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat).
Included in the TIP2k-Module.||31000-01|
|Microsyringe\500 mm3\TIP2k||Microsyringe\500 mm3\TIP2k: Microinjection syringe for Titration-Injection microPump, 500 mm3 (µl), fixed injection needle with rounded tip, with spacers.||31210-01|
|Microsyringe\200 mm3\TIP2k||Microsyringe\200 mm3\TIP2k: Microinjection syringe for Titration-Injection microPump, 200 mm3 (µl), fixed injection needle with rounded tip, with spacers.
Four units of this item are standard components of the TIP2k-Module.||31220-01|
|TIP2k-Needle Spacer||TIP2k-Needle Spacers for microinjection TIP2k needle, silicone stops (200/Pkg.) with mounting tool.
This item is a standard component of the TIP2k-Module.||31310-01|
|TIP2k Filter Papers||TIP2k Filter Papers (package of 10) to be used for the stoppers during TIP2k titration for Instrumental oxygen background correction to avoid constant manual siphoning off of extruded liquid.
One package is a standard component of the TIP2k-Module.||31320-01|
|TIP2k-Needle Safety Support with cable guide||TIP2k-Needle Safety Support with cable guide: for safe storage of TIP2k-needles when not required during the experiment.
This item is a standard component of the TIP2k-Module, O2k-TPP+ ISE-Module, O2k-pH ISE-Module.||31330-02|
|O2k-Chamber sV||O2k-Chamber sV: 12 mm inner diameter, Duran glass polished, with standard operation volume (V) of 0.5 cm3 (0.5 mL).
Two units of this item are standard components of the O2k-sV-Module.||33100-01|
|Stirrer-Bar sV\white PVDF\11.5x6.2 mm||Stirrer-Bar sV\white PVDF\11.5x6.2 mm, operated in the O2k-Chamber sV at constant stirring speed (standard is 750 rpm, or 12.5 Hz), to provide optimum mixing of the sample in the aqueous medium and ensure a stable signal of the polarographic oxygen sensor (OroboPOS) placed in a position of maximum current of the medium.
Two units of this item are standard components of the O2k-Chamber sV.||33210-01|
|Stopper sV\black PEEK\conical Shaft\central Port||Stopper sV\black PEEK\conical Shaft\central Port: with conical shaft and one central capillary (with PTFE, graphite, carbon fiber), Volume-Calibration Ring sV (A or B) for volume adjustment 0.5 ml; 2 mounted O-rings (O-ring sV\Viton\9.5x1 mm).
Two units of this item are standard components of the O2k-sV-Module.||34000-01|
|O-ring sV\Viton\9.5x1 mm||O-ring sV\Viton\9.5x1 mm, for PEEK Stopper sV, 2 are mounted on each PEEK Stopper sV, box of 8 as spares.
Three spare boxes of this product are standard components of the O2k-sV-Module.||34310-02|
|Volume-Calibration Ring sV||Volume-Calibration Ring sV (A or B), white PVDF, attached to PEEK O2k-Stopper sV.||3432A-01 or 3432B-01|
|O2k-Catalogue: O2k-Modules||4#### O2k-Catalogue: O2k-Modules. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user.||4####||4####|
|O2k-Catalogue: TPP+ ISE-Module||42### O2k-Catalogue: TPP+ ISE-Module||42###||42###|
|ISE Package 1 TPP or Ca||O2k-TPP+ and Ca2+ ISE\1 Chamber: ISE-Package for 1 TPP+ and Ca2+ electrode.||42010-01|
|Stopper\black PEEK\angular Shaft\side+6.2+2.6 mm Port||Stopper\black PEEK\angular Shaft\side+6.2+2.6 mm Port, for application with ISE; side titration port and two additional holes (6.2 mm and 2.6 mm); angular bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm).
Two stoppers are included in O2k-TPP+ ISE-Module.||42100-01|
|Stopper-Needle||Stopper-Needle: Short needle for bubble extrusion from port of the ISE-stopper.||42120-01|
|Ion-Selective Electrode TPP+ and Ca2+||Ion-Selective Electrode TPP+ and Ca2+: ISE with 6 mm outer diameter shaft, for Stopper\white PVDF\angular Shaft\side+6.2+2.6 mm Port.
O2k-TPP+ ISE-Module: 2 ISE.||42200-01|
|ISE-Inner Glass Electrode||ISE-Inner Glass Electrode of ISE, with Ag/AgCl- and Pt-wire||42212-01|
|ISE-Membrane Seal||ISE-Membrane Seal for Ion-Selective Electrode TPP+ and Ca2+.||42213-01|
|ISE-Compressible Tube||ISE-Compressible Tube for Ion-Selective Electrode TPP+ and Ca2+.||42214-01|
|ISE-Consumables Spares Set||ISE-Consumables Spares Set||42250-01|
|ISE-TPP+ Membranes||ISE-TPP+ Membranes, PVC, 4 mm diameter, box of 5 membranes.
O2k-TPP+ ISE-Module: 2 boxes per electrode.||42270-01|
|ISE-Ca2+ Membranes||ISE-Ca2+ Membranes: PVC, 4 mm diameter, box of 5 membranes.
To be used with the O2k-TPP+ ISE-Module. The ISE-Ca2+ Membranes have to be ordered separately.||42280-01|
|ISE-Membrane Mounting Tool||ISE-Membrane Mounting Tool for Ion-Selective Electrode TPP+ and Ca2+.
O2k-TPP+ ISE-Module: mounting tool included.||42300-01|
|ISE-Filling Syringe||ISE-Filling Syringe with needle for Ion-Selective Electrode TPP+ and Ca2+.||42310-01|
|Reference-Electrode\2.4 mm||Reference-Electrode\2.4 mm: 2.4 mm diameter glass shaft, for ISE.||42400-01|
|Replacement-Barrel||Replacement-Barrel for Reference-Electrode\2.4 mm, 2.4 mm diameter glass.||42401-01|
|Electrolyte\Reference-Electrode||Electrolyte\Reference-Electrode for Reference-Electrode\2.4 mm||42402-01|
|O2k-Catalogue: pH-Electrode||43### O2k-Catalogue: pH-Electrode||43###||43###|
|Stopper\black PEEK\conical Shaft\central+2.3+2.6 mm Port||Stopper\black PEEK\conical Shaft\central+2.3+2.6 mm Port: for pH and reference electrode, central titration port and two additional ports (2.3 mm and 2.6 mm); conical bottom; including Volume-Calibration Ring (A or B), 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm). Two stoppers are included in the O2k-pH ISE-Module.||43100-01|
|PH-Glass electrode||pH-Glass electrode with BNC plug. Two pH electrodes are included in the O2k-pH ISE-Module.||43200-01|
|O2k-Catalogue: O2k-Fluorescence||44### O2k-Catalogue: O2k-Fluorescence||44###||44###|
|Fluorescence-Sensor||Fluorescence-Sensor: LED with specified wavelength, photodiode, Filter-Cap attached with specific optical filter for the LED and/or photodiode. See Fluorescence-Sensor Green and Fluorescence-Sensor Blue.||442##|
|Fluorescence-Sensor Green||Fluorescence-Sensor Green: excitation LED 525 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set AmR for Amplex UltraRed measurements when delivered.
Two units of this item are standard components of the O2k-Fluo LED2-Module.||44210-01|
|Fluorescence-Sensor Blue||Fluorescence-Sensor Blue: excitation LED 465 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set Saf for measurement of mitochondrial membrane potential with Safranin when delivered. Filter sets for Magnesium green® / Calcium green® measurements are included.
Two units of this item are standard components of the O2k-Fluo LED2-Module.||44220-01|
|Filter-Cap||Filter-Cap: O2k-Fluo LED2-Module (O2k-Series D to G) sensors (Fluorescence-Sensor Green and Fluorescence-Sensor Blue) and O2k-FluoRespirometer (O2k-Series H) sensors (Smart Fluo-Sensor Green and Smart Fluo-Sensor Blue) are equipped with a removable Filter-Cap for exchange of optical filters for the optical pathways from the LED to the sample and from the sample to the photodiode.||44310-01|
|Filter Set AmR||Filter Set AmR: Set of filters for the determination of H2O2 production with Amplex UltraRed. These filters should be used together with Fluorescence-Sensor Green. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One pair of these filters is attached to Fluorescence-Sensor Green when delivered as part of the O2k-Fluorescence LED2-Module.
One filter set is a standard component of the O2k-Fluorescence LED2-Module and O2k-FluoRespirometer.||44321-01||
Excitation spectrum obtained with the Fluorescence-Sensor Green at 1mA current supply and equipped with the Filter Set AmR.
|Filter Set Saf||Filter set Saf: Set of filters for the (qualitative) determination of mitochondrial membrane potential with Safranin. These filters should be used together with Fluorescence-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One pair of these filters is attached to Fluorescence-Sensor Blue when delivered as part of the O2k-Fluorescence LED2-Module.
One filter set is a standard component of the O2k-Fluorescence LED2-Module and O2k-FluoRespirometer.
Excitation spectrum obtained with the Fluorescence-Sensor Blue at 1mA current supply and equipped with the Filter Set Saf.
|Filter Set MgG / CaG||Filter set MgG / CaG: Set of filters for the determination of concentraions of Mg2+ or Ca2+ with the fluorophores Magnesium green and Calcium green, respectively. These filters should be used together with Fluorescence-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).
One filter set is a standard component of the O2k-Fluorescence LED2-Module and O2k-FluoRespirometer.||44323-01||
Excitation spectrum obtained with the Fluorescence-Sensor Blue at 1mA current supply and equipped with the filter set for Magnesium green® / Calcium green®.
|O2k-Catalogue: NO-Amp-Module||46### O2k-Catalogue: NO-Amp-Module||46###||46###|
|Stopper\black PEEK\conical Shaft\central Port\peripheral 2.3 mm Port||Stopper\black PEEK\conical Shaft\central+peripheral 2.3 mm Port: for potentiometric O2k-MultiSensor applications (NO, H2S, H2O2); 1 additional port (2.3 mm); central titration port; conical bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm).
O2k-MultiSensor: two stoppers in O2k-NO Amp-Module.||46100-01|
|O2k-Catalogue: O2k-Accessories and Auxiliary Tools||5#### O2k-Catalogue: O2k Accessories and Auxiliary Tools||5####||5####|
|O2k-Catalogue: Titration-Tools||51### O2k-Catalogue: Titration-Tools||51###||51###|
|Microsyringe\10 mm3 51/0.13 mm||Hamilton Microsyringe\10 mm3 51/0.13 mm for manual titrations, 10 mm3 volume; fixed injection needle with rounded tip: 51 mm length, 0.13 mm inner diameter.
O2k-FluoRespirometer and Startup O2k-Respirometer: 6 included in the O2k-Titration Set.||51010-01|
|Plunger\10 mm3||Plunger\10 mm3 for Microsyringe\10 mm3 51/0.13 mm, spare.
O2k-FluoRespirometer and Startup O2k-Respirometer: 3 in O2k-Titration Set.||51011-01|
|Microsyringe\25 mm3 51/0.15 mm||Hamilton Microsyringe\25 mm3 51/0.15 mm for manual titrations, 25 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.15 mm inner diameter.
O2k-FluoRespirometer and Startup O2k-Respirometer: 6 in O2k-Titration Set.||51025-01|
|Microsyringe\50 mm3 51/0.15 mm||Hamilton Microsyringe\50 mm3 51/0.15 mm for manual titrations, 50 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.15 mm inner diameter.
O2k-FluoRespirometer and Startup O2k-Respirometer: 2 in O2k-Titration Set.||51050-01|
|Microsyringe\100 mm3 51/0.41 mm||Hamilton Microsyringe\100 mm3 51/0.41 mm for manual titrations, 100 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.41 mm inner diameter. It is recommended for injections of suspensions of isolated mitochondria. O2k-FluoRespirometer and Startup O2k-Respirometer: 1 included in the O2k-Titration Set.||51100-01|
|Syringe\500 mm3 51/0.41 mm||Hamilton Syringe\500 mm3 51/0.41 mm for manual titrations, 500 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.41 mm inner diameter; for injections of suspensions of isolated mitochondria and filling of the Microsyringe\200 mm3\TIP2k.
O2k-FluoRespirometer and Startup O2k-Respirometer: 1 included in the O2k-Titration Set.||51500-01|
|Syringe Racks||Syringe Racks: stainless steel; for proper placement of eight Hamilton microsyringes in HRR experiments; package of two (for a total of 16 microsyringes).
One package of two Syringe Racks and two Tube Racks is a standard component of the O2k-Titration Set.||51610-01|
|Syringe Collars||Syringe Collars (package of 20) for Hamilton microsyringes: for correctly storing the microsyringes in the Syringe Racks.
One package is a standard component of the Syringe Racks.||51611-01|
|Tube Racks||Tube Racks: stainless steel, accomodating four 50-ml tubes, for cleaning of microsyringes, cleaning and storage of stoppers during cleaning of the O2k-Chambers; package of two (for a total of eight tubes).
One package of two Tube Racks and two Syringe Racks is a standard component of the O2k-Titration Set.||51620-01|
|Syringe Storage Box||Syringe Storage Box: for storing the Hamilton microsyringes; incl. Syringe Labels, MiPNet19.14 SOP Hamilton microsyringes and MiPNet09.12 O2k-Titrations.
One item is a standard component of the O2k-Titration Set.||51630-01|
|Syringe Labels||Syringe Labels: set of labels with standard abbreviations (see O2k-Titrations).
One set of labels is a standard component of the Syringe Storage Box.||51631-02|
|Titration Set Upgrade||The Titration Set Upgrade consists of the new items of the O2k-Titration Set for upgrading your original set (consisting only of Hamilton microsyringes). The upgrade consists of the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks including tubes as well as an additional syringe (1 x 100 mm3).||51910-01||
|O2k-Catalogue: PBI-Shredder||52### O2k-Catalogue: PBI-Shredder||52###|
|PBI-Shredder SG3||PBI-Shredder SG3 for tissue homogenate preparation, heavy duty high torque SG3 driver with convertible handle, SG3 base with 3 position force setting lever (FSL), battery charger and two lithium ion batteries. The PBI-Shredder SG3 is included in the PBI-Shredder O2k-Set. Select 230 V or 120 V.
Oroboros Instruments: world-wide distributor.||52100|
|Shredder-Tubes||Shredder-Tubes: consisting of Shredder Tube FT500-PS with Lysis Disk, serrated Shredder-Ram and Shredder-Screw Cap, coral colour (Box of 100) and Shredder Tube FT500-PMS with Lysis Disk, serrated Shredder-Ram and Shredder-Screw Cap, coral colour (Box of 100). There is no difference between the white and the coral shredder tubes, except that the white tubes are also approved for high pressure. 1 box of each is included in the PBI-Shredder HRR-Set.||52210-01, 52220-01|
|O2k-Catalogue: Microbalance Mettler-Toledo||53### O2k-Catalogue: Microbalance Mettler-Toledo||53###|
|Microbalance 120 g||Microbalance 41 g to 120 g; 0.01 mg / 0.1 mg display; particularly for wet weight determination of permeabilized fibres.||53100-01|
|Microbalance-Transport Case||Microbalance transport case, not suitable for shipping||53300-01|
|O2k-Catalogue: O2k-Dissection Tools||54### O2k-Catalogue: O2k-Dissection Tools||54###||54###|
|Forceps\stainless Steel\straight Tip\sharp||Forceps\stainless Steel\straight Tip\sharp: for tissue preparation, stainless steel, antimagnetic. One pair is recommended for insertion of the sample into the O2k-Chamber and for handling in combination with Forceps\stainless Steel\rounded Tip\sharp.
Set: in O2k-Dissection Set and PBI-Shredder O2k-Set.||54210-01|
|Forceps\stainless Steel\rounded Tip\sharp||Forceps\stainless Steel\reounded Tip\sharp: for tissue preparation, stainless steel, antimagnetic. One pair is recommended for placing the tissue sample onto the microbalance and for handling in combination with Forceps\stainless Steel\straight Tip\sharp.
Set: in O2k-Dissection Set.||54220-01|
|Forceps\stainless Steel\angular Tip\fine||Forceps\stainless Steel\angular Tip\fine: for tissue preparation, stainless steel. Two pairs are used particularly for muscle fibre separation.
Set: 2 in O2k-Dissection Set.||54230-01|
|Scissors\stainless steel\straight Tip\sharp||Scissors\stainless steel\straight Tip\sharp, included in PBI-Shredder O2k-Set and O2k-Dissection Set.||54310-01|
|Motic Microscope||Motic Microscope SMZ-171 TP: for preparation of permeabilized fibres; compact and light stereo microscope, Greenough optical system, switching power supply for use worldwide (100-240V); including auxiliary ESD objective 2.0X(38.6mm).||54500-01|
|MiR05-Kit||Mitochondrial Respiration Medium - MiR05-Kit, 1 vial; for a final volume of 250 mL||60101-01|
|MitoKit-CII||Cell permeable compound - MitoKit-CII, 2 vials; 1 vial of plasma membrane-permeable succinate-nv (Snv; NV118; 25 mg) and 1 vial of plasma membrane-permeable malonate-nv (Mnanv; NV161; 10 mg).||60200-01|
|O2k-Workshops||Oroboros O2k-Workshops are organized as international oxygraph courses (IOC) on high-resolution respirometry since 1991. These range from four full-day training courses to conference lunch presentations of the Oroboros O2k and particularly the O2k-FluoRespirometer.||7####|
|MitoFit Coaching Days Innsbruck||
By organizing MitoFit Coaching Days Innsbruck, the Oroboros team responds to increasing demands for training in high-resolution respirometry (HRR) beyond the capacity of our regular O2k-Workshops. MitoFit Coaching Days are offered in the Oroboros MitoFit Laboratory according to demand for four to six participants (or labs). Experts provide training for quality control in high-resolution respirometry, aimed at standardization of SUIT protocols and fostering proficiency to generate experimental data of assured high quality as required for a data base on mitochondrial physiology.
The flexible programme is adjusted to the individual requirements of the participants, with specific coaching blocks particularly on instrumental setup and maintenance, instrumental quality control, MitoFit proficiency testing using standardized mitochondrial reference samples, DatLab analysis, SUIT protocols and experimental design, normalization of flux and data reporting, O2k-Fluorometry for measurement of hydrogen peroxide, measurement of mitochondrial membrane potential with fluorometric and potentiometric methods, measurement of respiration and acidification, quality control management in HRR. The MitoFit Coaching Days help participants to get prepared for their projects on HRR, even if the regular O2k-Workshops are fully booked.
|MitoFit Coaching Days - Basic||MitoFit Coaching Days - Basic: individual introductory training course (3 Days), standard registration, 4-6 participants.
One registration fee is waived in conjunction with the purchase of the Oroboros O2k (academic discount), valid during the O2k-Warranty Period.||70020-01|
|O2k-Workshop\4 Days\Standard||O2k-Workshop\4 Days\Standard: introductory O2k-Workshop and training course (4 Days), standard registration, basic or advanced.
Module: A registration fee is waived in conjunction with the purchase of the Oroboros O2k, valid during the O2k-Warranty Period.||70040-01|
|O2k-Workshop\4 Days\Student||O2k-Workshop\4 Days\Student: introductory training course (4 Days), student registration, basic or advanced.
Module: A registration fee is waived in conjunction with the purchase of the Oroboros O2k, valid during the O2k-Warranty Period.||70041-01|
|MitoFit Proficiency Test||The MitoFit Proficiency Test for high-resolution respirometry includes the user-friendly software DatLab 7.4 and the Oroboros Premium Customer Support. Upon successful completion of the test, a MitoFit Proficiency Certificate will be conferred from Oroboros Instruments acknowledging your personal proficiency in your laboratory environment. The standard package includes the MiR05-Kit and cryopreserved HEK 293T cells for the Proficiency Test for up to three participants from the same research group.||70050-01|
|Workshop 3 days Standard||O2k-Workshop (3 days), standard registration.||71003-01|
|Workshop 3 days Student||O2k-Workshop (3 days), student registration.||71003-02|
|Workshop 7 days Standard||O2k-MultiSensor Workshop (7 Days), standard registration; combined introductory and advanced O2k-MultiSensor Workshop.||71007-01|
|Workshop 7 days Student||O2k-Multisensor-Workshop (7 Days), student registration; combined introductory and advanced O2k-MultiSensor Workshop.||71007-02|
|O2k start-up service||O2k start-up service, O2k training course (3 days). In most cases, the O2k-Manual provides sufficient detail for setting up the O2k and starting measurements, particularly in conjunction with participation in an introductory O2k-Workshop. For specific requirements, however, a start-up visit by a scientific application specialist supports a highly efficient start of a project.||72003-01|
|O2k scientific support service||O2k scientific support visit (4 days) by an O2k-application specialist provides scientific support in specific areas of applications, including help with sample preparation, design and testing of titration protocols (including SUIT protocols), and DatLab analysis.||72004-01|
|OroboPOS-Connector Service||POS connector service; replacement of cable and amplifier in the OROBOROS electronics workshop (WGT).||80030-01|
|T-Shirt: MitoFit||T-Shirt MitoFit: Oroboros Logo on front, MitoFit Logo on back.||98000-01|
|T-Shirt: Oroboros black/organic cotton||T-Shirt Oroboros black/organic cotton: An Oroboros on the front.||98010-01|
|Packing\O2k-Box 1||Packing\O2k-Box 1: for shipment of the O2k-Main Unit, with polystyrene inlets. Keep the original packing material safely stored, for any future shipping purposes of the O2k-Main Unit.||99010-01|
|Packing\Peli Case||Packing\Peli Case: Watertight, crushproof, and dust proof case for safe transportation of the O2k:
Retractable extension handle, Strong polyurethane wheels with stainless steel bearings, Easy open Double Throw latches, Open cell core with solid wall design - strong, light weight, O-ring seal, Automatic Pressure Equalization Valve, Fold down handles, Stainless steel hardware and padlock protectors, 4 level Pick N Pluck™ with convoluted lid foam, Special foam inlets for O2k.
Interior Dimensions: 21.48" x 16.42" x 12.54" (54.5 x 41.7 x 31.8 cm)
|Packing\O2k-Box 2||Packing\O2k-Box 2, for shipment of accessories.||99020-01|