Ouabain

high-resolution terminology - matching measurements at high-resolution

Ouabain

Description

Ouabain (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.

Abbreviation: Oua

Reference: MiPNet03.02

Application in HRR

Oua: Ouabain octahydrate (≥95% (HPLC), powder; C₂₉H₄₄O_12*8H₂0); Sigma O 3125, store at R.T.; M = 728.77 g·mol^-1.

Caution: Light sensitive and toxic!

Preparation of 10 mM stock solution (dissolved in H₂O):

1. Weigh 7.3 mg ouabain.
2. Dissolve in 1 mL H₂O.
3. Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution.
4. Store in glass vials (recommendation) at -20 °C.

MitoPedia topics: Inhibitor