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Palmeira 2012 Methods Mol Biol

From Bioblast
Publications in the MiPMap
Palmeira CM, Rolo AP (2012) Mitochondrial membrane potential (ΔΨ) fluctuations associated with the metabolic states of mitochondria. Methods Mol Biol 810:89-101.

» PMID:22057562

Palmeira CM, Rolo AP (2012) Methods Mol Biol

Abstract: The study of the mitochondrial membrane potential (ΔΨ) is essential for an integrated appraisal of mitochondrial function, since it reflects differences in electrical potential and represents the main component of the proton electrochemical gradient accounting for more than 90% of the total available respiratory energy. Numerous methods have been used to estimate mitochondrial membrane potential (ΔΨ), including fluorescent methods and electrochemical probes. In this chapter, we describe several practical approaches that allow mitochondrial membrane potential (ΔΨ) evaluation, by using a tetraphenylphosphonium (TPP(+))-selective electrode. The main focus is given to the evaluation of ΔΨ in isolated mitochondria. Keywords: TPP+-selective electrode, membrane potential, mitochondria, permeabilized cells, metabolic states, permeability transition Bioblast editor: Sumbalova Z O2k-Network Lab: PT Coimbra Laranjinha J

Labels: MiParea: Instruments;methods 

Stress:Permeability transition, Oxidative stress;RONS  Organism: Rat  Tissue;cell: Liver  Preparation: Isolated mitochondria 

Regulation: ATP production, Calcium, mt-Membrane potential, Substrate, Uncoupler 

HRR: TPP, Theory