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Pichaud 2012 Comp Biochem Physiol A Mol Integr Physiol

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Publications in the MiPMap
Pichaud N, Rioux P, Blier PU (2012) In situ quantification of mitochondrial respiration in permeabilized fibers of a marine invertebrate with low aerobic capacity. Comp Biochem Physiol A Mol Integr Physiol 161:429-35.

ยป PMID:22244894

Pichaud N, Rioux P, Blier PU (2012) Comp Biochem Physiol A Mol Integr Physiol

Abstract: The aim of this study was to design a protocol to allow the assessment of normal and alternative pathways for electron transport in mitochondria using an in situ approach (on permeabilized fibers) in high-resolution respirometry. We measured the oxygen consumption of permeabilized fibers from Nereis (Neanthes) virens with different substrates and the presence of ADP. To estimate the alternative oxidase (AOX) activity, antimycin A was introduced in order to inhibit Complex III. Moreover, the apparent Complex IV (CIV) excess capacity was evaluated using different substrates to assess the implication of this complex in the partitioning of electrons during its progressive inhibition. Our in situ method enabled to quantify the activity of the normal CIV pathway as well as the AOX pathway when different substrates were oxidized by either CI, CII or both. Using this approach, we confirmed that according to the substrates used, each pathway has a different role and consequently is otherwise involved in the partitioning of electrons through the electron transport system, and suggested that the AOX activity is triggered not only by the redox state of the cell but also by the type of substrates provided to mitochondria. โ€ข Keywords: Nereis (Neanthes) virens (sandworm); alternative oxidase (AOX)

โ€ข O2k-Network Lab: CA Rimouski Blier PU, CA Moncton Pichaud N


Organism: Annelids 

Preparation: Permeabilized tissue 

Pathway: N, S, NS  HRR: Oxygraph-2k