Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Podrabsky 2000 Am J Physiol Regul Integr Comp Physiol

From Bioblast
Publications in the MiPMap
Podrabsky JE, Javillonar C, Hand SC, Crawford DL (2000) Intraspecific variation in aerobic metabolism and glycolytic enzyme expression in heart ventricles. Am J Physiol Regul Integr Comp Physiol 279:R2344-8.

Β» PMID: 11080103 Open Access

Podrabsky JE, Javillonar C, Hand SC, Crawford DL (2000) Am J Physiol Regul Integr Comp Physiol

Abstract: A previous phylogenetic analysis among 15 taxa of the teleost fish Fundulus suggested that there should be thermal-adaptive differences in heart metabolism among populations. To test this hypothesis, the rate of oxygen consumption and the activities of all 11 glycolytic enzymes were measured in isolated heart ventricle from two populations of Fundulus heteroclitus. Heart ventricular metabolism is greater in a northern population versus a southern population of these fish. Analysis of the amount of glycolytic enzymes indicates that 87% of the variation in cardiac metabolism within and between populations is explained by the variation in three enzymes (pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase, and lactate dehydrogenase). These enzymes are the same three enzymes that were predicted to be important based on previously determined phylogenetic patterns of expression. Our data indicate that near-equilibrium enzymes, as well as classically defined rate-limiting enzymes, can also influence metabolism. β€’ Keywords: Evolution, Cardiac adaptation

β€’ O2k-Network Lab: US FL Miami Crawford DL, US LA Baton Rouge Hand SC


Organism: Fishes  Tissue;cell: Heart