Porter 2015 Med Sci Sports Exerc

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Porter C, Reidy PT, Bhattarai N, Sidossis LS, Rasmussen BB (2015) Resistance exercise training alters mitochondrial function in human skeletal muscle. Med Sci Sports Exerc 47:1922-31.

» PMID: 25539479

Porter C, Reidy PT, Bhattarai N, Sidossis LS, Rasmussen BB (2015) Med Sci Sports Exerc

Abstract: Loss of mitochondrial competency is associated with several chronic illnesses. Therefore, strategies that maintain or increase mitochondrial function will likely be of benefit in a number of clinical settings. Endurance exercise has long been known to increase mitochondrial function in skeletal muscle. Comparatively little is known regarding the impact of resistance exercise training on skeletal muscle mitochondrial respiratory function.

The purpose of the current study was to determine the impact of chronic resistance training on skeletal muscle mitochondrial respiratory capacity and function.

Here, we studied the impact of a 12-week resistance exercise training program on skeletal muscle mitochondrial function in eleven young healthy men. Muscle biopsies were collected before and after the 12-week training program and mitochondrial respiratory capacity determined in permeabilized myofibers by high-resolution respirometry.

Resistance exercise training increased lean body mass and quadriceps muscle strength by 4 and 15%, respectively (p<0.001). Coupled mitochondria respiration supported by Complex I, and Complex I&II substrates, increased by 2- and 1.4-fold, respectively (p<0.01). The ratio of coupled Complex I supported respiration to maximal respiration increased with resistance exercise training (p<0.05), as did Complex I protein abundance (p<0.05), while the substrate control ratio for succinate was reduced after resistance exercise training (p<0.001). Transcripts responsible for proteins critical to electron transfer and NAD production increased with training (p<0.05), while transcripts involved in mitochondrial biogenesis were unaltered.

Collectively, 12-weeks of resistance exercise training resulted in qualitative and quantitative changes in skeletal muscle mitochondrial respiration. This adaptation occurs with modest changes in mitochondrial proteins and transcript expression. Resistance exercise training appears to be a means to augment the respiratory capacity and intrinsic function of skeletal muscle mitochondria.

O2k-Network Lab: US TX Galveston Porter C

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue  Enzyme: TCA cycle and matrix dehydrogenases 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS  HRR: Oxygraph-2k 


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