Putzer 1985 Comp Biochem Physiol
|Putzer V, Gnaiger E, Lackner R (1985) Flexibility of anaerobic metabolism in aquatic oligochaetes (Tubifex sp.). Biochemical and calorimetric changes induced by a deproteinized hydrolysate of bovine blood. Comp Biochem Physiol 82A:965-70.|
Abstract: Anaerobic processes were studied in Tubifex and other aquatic invertebrates. After the aerobic-anoxic transition of Tubifex, succinate accumulated up to about 25 μmol/g Wd within the first hour of anoxia, but steady-state levels were established after 4hr at only 10 μmol/g Wd in an open-flow system.
Proprionate accumulated after a lag of 30 min and reached steady-state concentrations of about 30 μmol/g Wd after 5 h of anoxia.
Lactate concentrations did not increase above 4 μmol/g Wd under anoxia. Its accumulation was not induced by exposure to a blood extract, although Tubifex has the potential for lactate production.
The initial rates of glycolytic endproduct accumulation were increased in the presence of the deproteinized blood extract by 60% (succinate, 0–30 min anoxia) and by 50% and 90% (proprionate, 30–60 min anoxia; 1% and 2% blood extract, respectively). The maximum and steady state levels of these metabolites were not influenced by the hydrolysate of blood.
Aerobic and anaerobic heat dissipation of Lumbriculus variegatus was stimulated by the addition to the medium of a deproteinized hydrolysate of bovine blood. Oxygen uptake of Cyclops abyssorum increased similarly upon the addition of the blood extract under hypoxia.
• Keywords: Twin-Flow
• O2k-Network Lab: AT Innsbruck Gnaiger E
Labels: MiParea: Respiration, Pharmacology;toxicology
Stress:Ischemia-reperfusion Organism: Crustaceans, Annelids
Preparation: Intact organism
Coupling state: ROUTINE