Rauchova 2003 Acta Biochim Pol

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Rauchová H, Drahota Z, Rauch P, Fato R, Lenaz G (2003) Coenzyme Q releases the inhibitory effect of free fatty acids on mitochondrial glycerophosphate dehydrogenase. Acta Biochim Pol 50:405-13.

» PMID: 12833166 Open Access

Rauchova H, Drahota Z, Rauch P, Fato R, Lenaz G (2003) Acta Biochim Pol

Abstract: Data presented in this paper show that the size of the endogenous coenzyme Q (CoQ) pool is not a limiting factor in the activation of mitochondrial glycerophosphate-dependent respiration by exogenous CoQ3, since successive additions of succinate and NADH to brown adipose tissue mitochondria further increase the rate of oxygen uptake. Because the inhibition of glycerophosphate-dependent respiration by oleate was eliminated by added CoQ3, our data indicate that the activating effect of CoQ3 is related to the release of the inhibitory effect of endogenous free fatty acids (FFA). Both the inhibitory effect of FFA and the activating effect of CoQ3 could be demonstrated only for glycerophosphate-dependent respiration, while succinate- or NADH-dependent respiration was not affected. The presented data suggest differences between mitochondrial glycerophosphate dehydrogenase and succinate or NADH dehydrogenases in the transfer of reducing equivalents to the CoQ pool.

Keywords: Mitochondrial glycerophosphate dehydrogenase, Free fatty acids, Coenzyme Q, Brown adipose tissue, Syrian hamster

O2k-Network Lab: CZ Prague Houstek J, CZ Hradec Kralove Cervinkova Z, IT Bologna Lenaz G, IT Bologna Genova ML


Labels: MiParea: Respiration 


Organism: Other mammals  Tissue;cell: Fat  Preparation: Isolated mitochondria 

Regulation: Substrate, Fatty acid 

Pathway: N, S, Gp, Other combinations  HRR: Oxygraph-2k