Respiratory states of mitochondrial preparations and living cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling-control state and the electron-transfer-pathway state.
Reference: Gnaiger 2020 MitoPathways
Communicated by Gnaiger E 2010-10-21, edited 2016-08-26. Edited by Doerrier C 2020-04-23.
Coupling control states
- Coupling control states and CCR of mitochondrial preparations:
- OXPHOS, LEAK respiration, ET-pathway (P, L, E) - corrected for ROX. CCR: L/E, P/E, L/P
- Coupling control states of living cells:
- ROUTINE, LEAK respiration, ET-pathway (R, L, E) - corrected for ROX. CCR: L/E, R/E, L/R
- Electron-transfer-pathway state, are defined by substrate type (at saturating concentration):
- Living cells: endogenous, exogenous substrate control
- Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry through Complex I (NADH pathway), CII (succinate pathway), or other branches converging at the Q-junction, particularly with fatty acid oxidation (fatty acid oxidation pathway control state), alpha-glycerophosphate, or substrate combinations applied for reconstitution of TCA cycle function (e.g. NS-pathway control state, etc.).
- Control by substrate concentration: Kinetic control states:
- Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
- Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.
Classical respiratory states
- Chance and Williams (1955):
- Derived respiratory states:
- Thermodynamics of irreversible processes:
MitoPedia: Respiratory states
- » See the complete MitoPedia: Respiratory states
- » MitoPedia: SUIT
MitoPedia concepts: MiP concept, Respiratory state, SUIT concept, Recommended
MitoPedia methods: Respirometry, Spectrophotometry
MitoPedia topics: EAGLE