Rodriguez 2022 Abstract Bioblast
Event: Bioblast 2022
Mitochondrial function depends on direct interactions between respiratory proteins encoded by genes in two genomes, mitochondrial and nuclear, which evolve in very different ways. Serious incompatibilities between these genomes can have severe effects on development, fitness and viability. The effect of subtle mitonuclear mismatches has received less attention, especially when subject to mild physiological stress. We investigate how various stressors affect phenotypic traits, mitochondrial function, metabolic pathways and gene expression in Drosophila larvae, and adults of both sexes. Flies fed either a high protein diet, the glutathione precursor N-acetyl cysteine (NAC), or the NADH precursor nicotinamide riboside (NR) and had sex, life-stage, and genotype-specific responses to these stressors. Metabolomic results point to changes in TCA cycle flux, while respirometry analysis shows changes in substrate use and complex I function. Our results support the notion that subtle mitonuclear mismatches can lead to diverging responses to mild physiological stress, undermining fitness in some cases, but surprisingly improving outcomes in other mismatched fly lines.
• Keywords: mitonuclear interactions, Drosophila melanogaster, larvae, diet, stress
- Department of Genetics, Evolution and Environment, University College London - [email protected]
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