Sharaf 2015 Abstract IOC106

Sharaf MS, Van den Heuvel MR, Stevens D, Kamunde C (2015) Mechanisms of mitochondrial import and interactions of zinc and calcium. Mitochondr Physiol Network 20.10.

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Sharaf MS, Van den Heuvel MR, Stevens D, Kamunde C (2015)

Event: IOC106 Schroecken

Zinc and calcium have highly interwoven functions that are essential for cellular homeostasis. Here, we studied the mechanisms of their import into mitochondria and interactions on oxidative phosphorylation (OXPHOS) and membrane potential (∆Ψm). We showed that while the two cations permeated the mitochondrial inner membrane via different mechanisms they synergistically inhibited OXPHOS but antagonistically dissipated ∆Ψm. Ruthenium red completely prevented calcium-induced OXPHOS inhibition suggesting that exclusive entry of calcium into the matrix via mitochondrial calcium uniporter is a fundamental step for the functional impairment caused by this cation. Overall, these data indicate that interactions of zinc and calcium on mitochondrial function result from mechanisms other than competition for their uptake pathways.

• Keywords: Zinc, Calcium, Mitochondria, Mitochondrial respiration, Mitochondrial membrane potential, MCU

• O2k-Network Lab: CA Charlottetown Kamunde C

Labels: MiParea: Respiration, mt-Membrane 

Regulation: Calcium 

Affiliations and Support

Dept Biomed Sc, Atlantic Veterinary College, Univ Prince Edward Island, Charlottetown, PE, Canada.