Shoubridge 2015 Abstract MiP2015
|Posttranscriptional regulation of mitochondrial gene expression.|
Regulation of the translation and turnover of mRNAs is central to the control of gene expression. Eukaryotes have evolved mechanisms to sequester mRNAs and their associated RNA-binding proteins into non-membrane delimited bodies called RNA granules as a mechanism to control these processes and to respond to changing cellular demands and physiological stresses. We recently identified mitochondrial RNA granules, and showed that they contain newly synthesized mitochondrial RNA and the RNA-binding protein GRSF1, suggesting that compartmentalization of mitochondrial RNAs might also be important for mitochondrial gene expression. Silencing of GRSF1 resulted in dysregulation of mitochondrial transcription, aberrant loading of mRNAs and lncRNAs onto mitochondrial ribosomes, and compromised mitochondrial ribosome biogenesis. We have further characterized the mitochondrial RNA granule proteome, and find that it contains a large toolbox of proteins dedicated to RNA metabolism including proteins involved in transcript processing, poly(A) addition, rRNA and tRNA modification, mRNA degradation, and ribosome biogenesis. I will discuss recent developments in this area and the relevance to mechanisms of mitochondrial disease.
Labels: MiParea: mt-Biogenesis;mt-density, mtDNA;mt-genetics
Event: A1, Oral MiP2015
Montreal Neurological Inst, Genetics, Canada. - [email protected]