Soriano 2016 Abstract Mito Xmas Meeting Innsbruck

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Exploring the role of OPA1-MIC60 containing complexes in the regulation of cristae and cristae junctions biogenesis.


Glytsou C, Calvo E, Cogliati S, Mehrotra A, Anastasia I, Rigoni G, Raimondi A, Shintani N, Loureiro M, Vazquez J, Pellegrini L, Enriquez JA, Scorrano L, Soriano ME (2016)

Event: Mito Xmas Meeting 2016 Innsbruck AT

The mitochondrial cristae and cristae-inner boundary membrane junctions (CJ) architecture are controlled by the mitochondrial contact site and cristae organizing system (MICOS) and by Optic atrophy 1 (OPA1), but whether and how they interplay is unknown. Proteomics, native gel electrophoresis and immunoprecipitation indicates that the MICOS component MIC60 and OPA1 physically interact in mitochondrial high molecular weight complexes targeted during cristae remodeling. A combination of genetic epistatic analysis, electron microscopy and tomography, biochemistry and physiology places OPA1 upstream of MIC60 in the control of CJ number and stability, whereas OPA1 defines cristae and CJ width independently of MIC60. Accordingly, MIC60 does not control apoptotic cristae remodeling, cytochrome c redistribution and mitochondrial apoptosis. We provide a unifying model for mammalian cristae biogenesis where OPA1 independently specifies width of cristae and CJ, and MIC60 requires OPA1 to define CJ number and stability.

Labels: MiParea: mt-Biogenesis;mt-density 

Event: B2, Oral 


Glytsou C(1,2,3), Calvo E(4), Cogliati S(1,4), Mehrotra A(1), Anastasia I(1), Rigoni G(1), Raimondi A(5), Shintani N(1,3,6), Loureiro M(4), Vazquez J(4), Pellegrini L(7), Enriquez JA(4), Scorrano L(1,3), Soriano ME(1,3)
  1. Dept Biology, Univ Padova, Italy
  2. IRCCS Fondazione Santa Lucia, Rome, Italy
  3. Dulbecco-Telethon Inst, Venetian Inst Molecular Medicine, Padova, Italy
  4. Centro Nacional Investigaciones Cardiovasculares Carlos III, Madrid, Spain
  5. Centro Imaging Sperimentale, IRCCS Inst Scientifico San Raffaele, Milano, Italy
  6. Advanced Pharmaco-sc, Graduate School Pharmaceutical Sc, Osaka Univ, Japan
  7. Dept Molecular Biology, Medical Biochemistry Pathology, Univ Laval, Quebec, Canada