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Affourtit 2012 Methods Mol Biol
Additional label Amplex Red  +
Coupling states LEAK  +
Enzyme Complex II;succinate dehydrogenase  + , Complex III  +
Has abstract Oxidative phosphorylation is an important
Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton and electron leaks. In this chapter, methods are presented to measure proton and electron leak activities in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasised.
e oxidative phosphorylation is emphasised.  +
Has editor [[Sumbalova Z]]  +
Has info [https://www.ncbi.nlm.nih.gov/pubmed/22057567 PMID:22057567]  +
Has publicationkeywords mitochondria  + , oxygen consumption  + , membrane potential  + , reactive oxygen species production  + , oxidative phosphorylation  + , modular kinetic analysis  + , proton leak  + , electron leak  +
Has title Affourtit C, Quinlan CL, Brand MD (2012) Measurement of proton leak and electron leak in isolated mitochondria. Methods Mol Biol 810:165-82.  +
Instrument and method TPP  + , Theory  +
Mammal and model Rat  +
MiP area Respiration  +
Respiration and regulation mt-Membrane potential  +
Stress Oxidative stress;RONS  +
Tissue and cell Skeletal muscle  +
Was published in journal Methods Mol Biol +
Was published in year 2012  +
Was written by Affourtit C + , Quinlan CL + , Brand MD +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
11:23:12, 8 February 2018  +
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