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Afshordel 2014 Prostaglandins Leukot Essent Fatty Acids
Coupling states LEAK  + , OXPHOS  + , ET  +
Diseases Aging;senescence  +
Has abstract The present study investigated the effects
The present study investigated the effects of orally administered long chain omega-3 polyunsaturated fatty acids (PUFA) on mitochondrial function and processing of the amyloid precursor protein (APP) in brains of young (3 months old) and aged (24 months old) NMRI-mice. Neuroprotective properties of fish oil (FO) (1.6ml/kg p.o.) were assessed ex vivo after 21 days in dissociated brain cells (DBC) and isolated mitochondria. Docosahexaenoic acid (DHA) levels were significantly lower in blood and brains of aged mice which were compensated by FO administration. Isolated DBC and mitochondria from aged mice showed significantly lower adenosine triphosphate (ATP) levels and reduced activity of complexes I+II and IV of the mitochondrial respiration system, respectively. FO restored the age-related decrease in respiration and improved ATP production. Moreover, FO increased the levels of anti-apoptotic Bcl-2 protein. Cell membrane fractions isolated from the brain of aged mice exhibited lower membrane fluidity, which was partially improved under FO treatment. In comparison to young animals, levels of neuroprotective sAPPα were significantly lower in the brain of aged mice. However, levels of sAPPα, Aβ and C-terminal APP fragments (CTF) were largely unchanged after FO treatment in aged mice. Neuroprotectin D-1 (NPD-1) represents a neuroprotective compound that is derived from unesterified DHA. Levels of NPD1-like metabolites (NPD1-like) and of unesterified DHA were significantly increased in brains of aged mice. FO treatment further strongly increased NPD1-like levels indicating an accelerated conversion rate of free DHA to NPD1-like. Our findings provide new mechanisms underlying the neuroprotective actions of omega-3 PUFA and identified FO as a promising nutraceutical to delay age-related mitochondrial dysfunction in the brain.
ed mitochondrial dysfunction in the brain.  +
Has info [http://www.ncbi.nlm.nih.gov/pubmed/24972878 PMID: 24972878]  +
Has publicationkeywords Brain  + , Aging  + , Omega-3 fatty acids  + , Fish oil  + , Docosahexaenoicacid  + , Bioenergetics  +
Has title Afshordel S, Hagl S, Werner D, Roehner N,
Afshordel S, Hagl S, Werner D, Roehner N, Koegel D, Bazan NG, Eckert GP (2014) Omega-3 polyunsaturated fatty acids improve mitochondrial dysfunction in brain aging - impact of Bcl-2 and NPD-1 like metabolites. Prostaglandins Leukot Essent Fatty Acids 92:23-31.
andins Leukot Essent Fatty Acids 92:23-31.  +
Instrument and method Oxygraph-2k  +
Mammal and model Mouse  +
MiP area Respiration  +
Pathways N  + , S  + , CIV  + , NS  + , ROX  +
Preparation Isolated mitochondria  +
Tissue and cell Nervous system  +
Was published by MiPNetLab DE Giessen Eckert GP +
Was published in journal Prostaglandins Leukot Essent Fatty Acids +
Was published in year 2014  +
Was written by Afshordel S + , Hagl S + , Werner D + , Roehner N + , Koegel D + , Bazan NG + , Eckert GP +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
08:49:22, 27 March 2018  +
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