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Chowdhury 2010 Diabetes
Coupling states LEAK  + , OXPHOS  +
Diseases Diabetes  +
Has abstract '''Objective''': Impairments in mitochondr
'''Objective''': Impairments in mitochondrial physiology may play a role in diabetic sensory neuropathy. We tested the hypothesis that mitochondrial dysfunction in sensory neurons is due to abnormal mitochondrial respiratory function. '''Research design and methods''': Rates of oxygen consumption were measured in mitochondria from dorsal root ganglia (DRG) of 12- to- 22-week streptozotocin (STZ)-induced diabetic rats, diabetic rats treated with insulin, and age-matched controls. Activities and expression of components of mitochondrial complexes and reactive oxygen species (ROS) were analyzed. '''Results''': Rates of coupled respiration with pyruvate + malate (P + M) and with ascorbate + TMPD (Asc + TMPD) in DRG were unchanged after 12 weeks of diabetes. By 22 weeks of diabetes, respiration with P + M was significantly decreased by 31-44% and with Asc + TMPD by 29-39% compared with control. Attenuated mitochondrial respiratory activity of STZ-diabetic rats was significantly improved by insulin that did not correct other indices of diabetes. Activities of mitochondrial complexes I and IV and the Krebs cycle enzyme, citrate synthase, were decreased in mitochondria from DRG of 22-week STZ-diabetic rats compared with control. ROS levels in perikarya of DRG neurons were not altered by diabetes, but ROS generation from mitochondria treated with antimycin A was diminished compared with control. Reduced mitochondrial respiratory function was associated with downregulation of expression of mitochondrial proteins. '''Conclusions''': Mitochondrial dysfunction in sensory neurons from type 1 diabetic rats is associated with impaired rates of respiratory activity and occurs without a significant rise in perikaryal ROS.
hout a significant rise in perikaryal ROS.  +
Has info [ PMID: 20103706 Open Access]  +
Has publicationkeywords Dorsal root ganglia  + , Diabetes  +
Has title Chowdhury SK, Zherebitskaya E, Smith DR, A
Chowdhury SK, Zherebitskaya E, Smith DR, Akude E, Chattopadhyay S, Jolivalt CG, Calcutt NA, Fernyhough P (2010) Mitochondrial respiratory chain dysfunction in dorsal root ganglia of streptozotocin-induced diabetic rats and its correction by insulin treatment. Diabetes 59:1082-91.
by insulin treatment. Diabetes 59:1082-91.  +
Instrument and method Oxygraph-2k  +
Mammal and model Rat  +
MiP area Respiration  +
Pathways N  + , CIV  +
Preparation Isolated mitochondria  +
Stress Oxidative stress;RONS  + , Mitochondrial disease  +
Tissue and cell Nervous system  +
Was published by MiPNetLab CA Winnipeg Fernyhough P +
Was published in journal Diabetes +
Was published in year 2010  +
Was written by Roy Chowdhury SK + , Zherebitskaya E + , Smith DR + , Akude E + , Chattopadhyay S + , Jolivalt CG + , Calcutt NA + , Fernyhough P +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
17:10:07, 1 April 2018  +
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