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Gnaiger 2000 Life in the Cold
Coupling states OXPHOS  +
Has abstract Development of hibernation strategies for
Development of hibernation strategies for cold preservation of human organs represents a far-reaching goal in transplantation surgery. Short cold storage times of <6 h tolerated by the human heart remain a major clinical problem. Mitochondrial cold storage-reperfusion injury is becoming recognized as a limiting factor in preservation of organs from non-hibernating mammals. Damaged mitochondria lead to cellular injury by reduction of ATP supply, oxidative stress, disturbance of ion balance, cytochrome c release and induction of apoptosis and necrosis. Profiles of mitochondrial injuries differed after (1) cold preservation of isolated rat heart mitochondria, (2) cold preservation of the rat heart, and (3) after transplantation and rewarming/reperfusion. Importantly, a specific defect of complex I of the electron transport chain, uncoupling of oxidative phosphorylation and the pronounced release of cytochrome c from mitochondria were absent after cold storage but developed during reperfusion, in proportion to the loss of heart function. Cold preservation of isolated heart mitochondria could be significantly prolonged by a mitochondrial preservation solution containing antioxidants, mitochondrial substrates, ATP, histidine, and oncotic agents. Successful cold storage of heart mitochondria demonstrates a large scope for improvement of heart preservation solutions. In this context, comparison of intracellular conditions and cold ischemia-reperfusion injury in hibernating and non-hibernating mammals may provide a rationale for improvement of clinical organ hibernation strategies.
of clinical organ hibernation strategies.  +
Has info [[File:PDF.jpg|100px|link=http://www.bioblast.at/images/6/6e/Gnaiger_2000_Life_in_the_Cold.pdf |Bioblast pdf]]  +
Has publicationkeywords MiR05  + , [[MiR06]]  +
Has title [[Image:O2k-Protocols.jpg|right|80px|link=
[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols contents]] Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42.
nger, Heidelberg, Berlin, New York:431-42.  +
Instrument and method Oxygraph-2k  + , O2k-Protocol  +
Mammal and model Rat  +
MiP area Respiration  + , Instruments;methods  + , mt-Medicine  + , Pharmacology;toxicology  +
Preparation Permeabilized tissue  + , Isolated mitochondria  +
Respiration and regulation Cyt c  + , Ion;substrate transport  + , Substrate  + , Temperature  +
Stress Ischemia-reperfusion  +
Tissue and cell Heart  +
Was published by MiPNetLab AT Innsbruck Gnaiger E + , AT Innsbruck Oroboros +
Was published in journal Springer +
Was published in year 2000  +
Was written by Gnaiger E + , Kuznetsov AV + , Schneeberger S + , Seiler R + , Brandacher G + , Steurer W + , Margreiter R +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
15:18:35, 28 April 2019  +
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