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Gupte 2009 Diabetes
Coupling states OXPHOS  +
Diseases Diabetes  +
Enzyme Complex III  + , Complex IV;cytochrome c oxidase  +
Has abstract OBJECTIVE—Heat treatment and overexpressio
OBJECTIVE—Heat treatment and overexpression of heat shock protein 72 (HSP72) have been shown to protect against high-fat diet–induced insulin resistance, but little is known about the underlying mechanism or the target tissue of HSP action. The purpose of this study is to determine whether ''in vivo'' heat treatment can prevent skeletal muscle insulin resistance. RESEARCH DESIGN AND METHODS—Male Wistar rats were fed a high-fat diet (60% calories from fat) for 12 weeks and received a lower-body heat treatment (41°C for 20 min) once per week. RESULTS—Our results show that heat treatment shifts the metabolic characteristics of rats on a high-fat diet toward those on a standard diet. Heat treatment improved glucose tolerance, restored insulin-stimulated glucose transport, and increased insulin signaling in soleus and extensor digitorum longus (EDL) muscles from rats fed a high-fat diet. Heat treatment resulted in decreased activation of Jun NH2-terminal kinase (JNK) and inhibitor of kB kinase (IKK-β), stress kinases implicated in insulin resistance, and upregulation of HSP72 and HSP25, proteins previously shown to inhibit JNK and IKK-β activation, respectively. Mitochondrial citrate synthase and cytochrome oxidase activity decreased slightly with the high-fat diet, but heat treatment restored these activities. Data from L6 cells suggest that one bout of heat treatment increases mitochondrial oxygen consumption and fatty acid oxidation. CONCLUSIONS—Our results indicate that heat treatment protects skeletal muscle from high-fat diet–induced insulin resistance and provide strong evidence that HSP induction in skeletal muscle could be a potential therapeutic treatment for obesity induced insulin resistance [ diabetes].
/diabetes/diabetes-details.html diabetes].  +
Has info [ PMID: 19073766 Open Access]  +
Has publicationkeywords Heat shock protein 72 (HSP72)  + , Insulin  + , High-fat diet  + , Mitochondria  +
Has title Gupte AA, Bomhoff GL, Swerdlow RH, Geiger PC (2009) Heat treatment improves glucose tolerance and prevents skeletal muscle insulin resistance in rats fed a high-fat diet. Diabetes 58:567-78.  +
Instrument and method Oxygraph-2k  +
Mammal and model Rat  +
MiP area Respiration  + , Exercise physiology;nutrition;life style  +
Respiration and regulation ADP  + , Substrate  + , Temperature  + , Fatty acid  +
Tissue and cell Skeletal muscle  +
Was published by MiPNetLab US KS Kansas City Swerdlow RH +
Was published in journal Diabetes +
Was published in year 2009  +
Was written by Gupte AA + , Bomhoff GL + , Swerdlow RH + , Geiger PC +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
08:58:47, 3 December 2019  +
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