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Harris 2015 Diabetes
Additional label Labels  + , 2018-10  +
Coupling states OXPHOS  + , ET  +
Enzyme Complex I  + , Complex II;succinate dehydrogenase  + , Complex III  + , Complex IV;cytochrome c oxidase  + , Complex V;ATP synthase  +
Has abstract Perilipin 5 (PLIN5) is a lipid droplet pro
Perilipin 5 (PLIN5) is a lipid droplet protein and is highly expressed in oxidative tissue. Expression of the PLIN5 gene is regulated by peroxisome proliferator-activated receptor-α, fasting, and exercise. However, the effect of increased muscle PLIN5 expression on whole-body energy homeostasis remains unclear. To examine this, we developed a mouse line with skeletal muscle PLIN5 overexpression (MCK-Plin5). We show that MCK-Plin5 mice have increased energy metabolism and accumulate more intramyocellular triacylglycerol but have normal glucose and insulin tolerance. MCK-Plin5 mice fed high-fat chow manifest lower expression of inflammatory markers in their liver and increased expression of "browning" factors in adipose tissue. This muscle-driven phenotype is, at least in part, mediated by myokines; the MCK-Plin5 mice have 80-fold higher FGF21 gene expression in muscle and increased serum FGF21 concentration. The increase in FGF21 occurs mainly in muscles with a predominance of fast-twitch fibers, suggesting that fiber type-specific lipid storage may be part of the mechanism conferring metabolic protection in MCK-Plin5 mice. In conclusion, upregulating the PLIN5 level in skeletal muscle drives expression of the FGF21 gene in fast-twitch fibers and is metabolically protective. These findings provide insight into the physiology of PLIN5 and the potential contribution of its upregulation during exercise.
ution of its upregulation during exercise.  +
Has editor [[Plangger M]]  +
Has info [ PMID: 25829453 Open Access]  +
Has title Harris LA, Skinner JR, Shew TM, Pietka TA, Abumrad NA, Wolins NE (2015) Perilipin 5-driven lipid droplet accumulation in skeletal muscle stimulates the expression of fibroblast growth factor 21. Diabetes 64:2757-68.  +
Instrument and method Oxygraph-2k  +
Mammal and model Mouse  +
MiP area Respiration  + , Genetic knockout;overexpression  + , Exercise physiology;nutrition;life style  +
Pathways N  + , S  + , NS  + , ROX  +
Preparation Permeabilized tissue  +
Tissue and cell Skeletal muscle  +
Was published by MiPNetLab US MO St Louis Abumrad NA +
Was published in journal Diabetes +
Was published in year 2015  +
Was written by Harris LA + , Skinner JR + , Shew TM + , Pietka TA + , Abumrad NA + , Wolins NE +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
12:50:52, 18 October 2018  +
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