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Kuznetsov 2002 Anal Biochem
Coupling states LEAK  + , OXPHOS  +
Enzyme Marker enzyme  +
Has abstract Mitochondrial respiratory function was stu
Mitochondrial respiratory function was studied in permeabilized pig liver biopsies. The cell membrane was permeabilized mechanically in tissue samples of 2–7 mg, for application of a standardized substrate/inhibitor titration protocol in high-resolution respirometry. Specific respirometric tests demonstrated complete plasma membrane permeabilization and accessibility of substrates to intact mitochondria. High respiratory adenylate control ratios and cytochrome c conservation in the tissue preparation were comparable or even better than in isolated mitochondria. Citrate synthase and cytochrome c oxidase activities remained at 85% of controls after up to 98 h storage of liver tissue at 0°C in histidine–tryptophan–ketoglutarate solution. Multiple mitochondrial defects, however, were indicated after 48 h cold storage by the decline in respiratory capacity, which was lowered to a larger extent with complex I substrates compared to respiration with substrates for complex II or IV, measured in the absence of cytochrome c. After prolonged ischemia, the adenylate control ratio was significantly reduced, and cytochrome c depletion was detected by the stimulatory effect of cytochrome c. High-resolution respirometry allows the assessment of mitochondrial function in a few milligrams of permeabilized liver tissue, without isolation of mitochondria. This provides a basis for the analysis of mitochondrial function in human liver biopsies.
hondrial function in human liver biopsies.  +
Has info [http://www.ncbi.nlm.nih.gov/pubmed/12054447 PMID: 12054447]  +
Has publicationkeywords Mitochondrial respiration  + , Pig liver tissue  + , Cell membrane permeabilization  + , Cold ischemia  + , Mitochondrial injury  +
Has title Kuznetsov AV, Strobl D, Ruttmann E, Königsrainer A, Margreiter R, Gnaiger E (2002) Evaluation of mitochondrial respiratory function in small biopsies of liver. Anal Biochem 305:186-94.  +
Instrument and method Oxygraph-2k  +
Mammal and model Pig  +
MiP area Respiration  + , Instruments;methods  + , mt-Biogenesis;mt-density  + , mt-Medicine  +
Pathways N  + , S  + , CIV  + , ROX  +
Preparation Permeabilized tissue  +
Stress Ischemia-reperfusion  +
Tissue and cell Liver  +
Was published by MiPNetLab AT Innsbruck Gnaiger E + , AT Innsbruck Oroboros +
Was published in journal Anal Biochem +
Was published in year 2002  +
Was written by Kuznetsov AV + , Strobl D + , Ruttmann E + , Koenigsrainer A + , Margreiter R + , Gnaiger E +
Categories Publications
Modification date
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07:44:27, 23 January 2019  +
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