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MiPNet04.05 Titration-Injection
Additional label O2k-Demo  +
Has abstract '''Gnaiger E, Rieger G (1999) From step ti
'''Gnaiger E, Rieger G (1999) From step titration to ramp injection: Uncoupling by FCCP with TIP. Mitochondr Physiol Network 04.05.''' :» Product: [[O2k-Catalogue: TIP2k|TIP2k]], [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]] Fully supported by the O2k-Core and control of the TIP2k by the software DatLab: The TIP2k can be programmed for multiple titrations and continuous injections. As an alternative to traditional step titration, the TIP offers the new option of ramp injection, providing maximum resolution of the concentration dependence of oxygen flux. This is illustrated by the recording of cellular respiratory flux as a function of a continuous increase of uncoupler (FCCP) concentration. ''Titration:'' Programmable, automatic titration regimes, with titration volumes of 0.05 to 250 µl, variable titration intervals and duration of titration pulses. ''Injection:'' Steady-state injection: Operation at quasi steady-states by continuous injection of substrates at limiting rates of consumption, providing new flexibility in experimental design by combining the technical advantages of closed and open systems. Programmable injection flows: 0.01 to 25 µl.s-1. Ramp injection (MiPNet04.05, see above): Ramp increase of effector concentrations by "continuous titration". DatLab software for feedback control by the the TIP2k: for steady-state respirometry at selected oxygen levels and pH-stat applications.
ed oxygen levels and pH-stat applications.  +
Has info [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/8/8e/MiPNet04.05_Titration-Injection.pdf |Bioblast pdf]]  +
Has title [[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols contents]]From step titration to ramp injection: Uncoupling by FCCP with TIP.  +
Instrument and method Oxygraph-2k  + , TIP2k  + , O2k-Protocol  +
Mammal and model Human  +
MiP area Respiration  + , Instruments;methods  +
Preparation Intact cells  +
Respiration and regulation Uncoupler  +
Tissue and cell Endothelial;epithelial;mesothelial cell  + , HUVEC  +
Was published by MiPNetLab AT Innsbruck Oroboros +
Was published in journal Mitochondr Physiol Network +
Was published in year 1999-01  +
Was written by Oroboros +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
16:12:55, 5 August 2017  +
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