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Uploads by Krumschnabel Gerhard

From Bioblast

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File list
Date Name Thumbnail Size Description Versions
09:01, 15 July 2019 Signal stability.PNG (file) 65 KB   1
09:01, 15 July 2019 No response to dithionite.PNG (file) 71 KB   1
08:14, 15 July 2019 Different O2 levels and high zero.PNG (file) 77 KB   1
11:09, 5 July 2019 Saf excitation scan 1mA.png (file) 358 KB Excitation spectrum obtained with the Fluorescence-Sensor Blue at 1mA current supply and equipped with the Filter Set Saf. 1
11:09, 5 July 2019 MgG excitation scan 1mA.png (file) 372 KB Excitation spectrum obtained with the Fluorescence-Sensor Blue at 1mA current supply and equipped with the filter set for Magnesium green® / Calcium green®. 1
11:04, 5 July 2019 AmR excitation scan 1mA.png (file) 476 KB Excitation spectrum obtained with the Fluorescence-Sensor Green at 1mA current supply and equipped with the Filter Set AmR. 1
08:23, 26 April 2019 Rot-Ama intact cells.png (file) 74 KB HeLa cells examined with a simple Coupling Control Protocol. As in many cells, Rotenone inhibits respiration to a large degree and further addition of Antimycin A has only little further inhibitory effect. [Exp.: 2018-12-04 PS4-02.DLD] 1
07:53, 4 April 2019 Brine shrimp embryo mitochondria Malate effect.png (file) 75 KB   2
10:09, 22 August 2017 PX calibration 1.PNG (file) 2 KB   1
10:08, 22 August 2017 MultiSensor calibration pX.PNG (file) 2 KB   1
08:54, 22 August 2017 Amp calibration 2.PNG (file) 14 KB   1
08:52, 22 August 2017 Amp calibration 1.PNG (file) 2 KB   1
08:49, 22 August 2017 MultiSensor calibration.PNG (file) 2 KB   1
09:50, 21 August 2017 Stirrer test.png (file) 27 KB   1
08:33, 14 August 2017 Air calibration.png (file) 42 KB Oxygen concentration (blue plot; 180 µM; full scale 50 nmol/ml or 50 µM) over 1 h after switching on the Oxygraph-2k (Power-O2k P6, chamber A) and setting the experimental temperature at 37 °C, using medium stirred for equilibration with a gas phase... 1
07:43, 14 August 2017 Stopper-spacer.png (file) 242 KB   1
08:47, 3 July 2017 Fig2B-01.jpg (file) 1.72 MB Figure 2. Genotype and tissue-dependent effect of catalase or its impurities on CI&CII-linked respiration. (A) Isolated kidney mitochondria. (B) Isolated liver mitochondria. A magenta coloured line represents a respirometry trace from a wild type mouse... 1
08:43, 3 July 2017 Fig2A-01.jpg (file) 1.74 MB Figure 2. Genotype and tissue-dependent effect of catalase or its impurities on CI&CII-linked respiration. (A) Isolated kidney mitochondria. (B) Isolated liver mitochondria. A magenta coloured line represents a respirometry trace from a wild type mouse... 1
08:40, 3 July 2017 Fig1-01.jpg (file) 1.42 MB Figure 1. Inhibitory effect of catalase or its impurities on CI&CII-linked respiration in isolated mouse heart mitochondria. H2O2 injections (100nM) were used to calibrate simultaneous Amplex Red-HRP assay (data not shown). Contributiuon to the discus... 1
08:44, 23 August 2016 Low Omy in PLT.jpg (file) 118 KB Preventing the reduction of ETS by Omy using a lower Omy concentration. Example by Luiz Felipe Garcia e Souza using human platelets. 1
09:02, 16 August 2016 PX calibration window.JPG (file) 63 KB screenshot from DatLab7 for website 1
14:31, 12 August 2016 DL7graph select plots amp.jpg (file) 34 KB DL7 screen shot: Graph/select plots Amp raw signal selected 1
11:47, 13 November 2015 MG-63 cells digitonin permeabilization.png (file) 16 KB Original file: 2014-05-05 P2-01.DLD 1
11:39, 13 November 2015 C2C12 digitonin permeabilization.png (file) 35 KB   1
10:05, 13 November 2015 TMRM Imt mouse heart.png (file) 60 KB Isolated mouse cardiac mitochondria (prepared by C. Doerrier), measured with Macro Spinazzi during his short-term visit 2015-10-21/22. 1
09:07, 11 March 2015 TMRM permHEK293T.png (file) 48 KB 2015-02-25 P7-02.DLD Measurement of mtMP with Marielle Hansl using cultured HEK 393T cells. 1
14:16, 2 March 2015 MiPNet14.05 TPP-MitoMembranePotential.png (file) 1.35 MB higher resolution version from Zuzana Sumbalova 1
10:39, 19 December 2014 MiPNet19 22 Isolation of rat heart mitochondria.pdf (file) 146 KB   1
14:46, 17 October 2014 TMRM Thom rat liver.png (file) 64 KB chamber A from file 2014-09-26 P5-02_TMRM_GK.DLD Thom from rat liver obtained from Zuzana Sumbalova. 1
13:45, 22 September 2014 Respirometric media.png (file) 4 KB   1
11:52, 7 August 2014 Omy Yeast.png (file) 159 KB No inhibition of respiration in yeast with 10 µM Omy. Experiment provided by Gennaro Agrimi (2014-08-06). 1
08:52, 10 July 2014 Abstract Bednarczyk P Graphical.jpg (file) 477 KB   1
13:57, 11 March 2014 TMRM Fig 11.png (file) 24 KB Figure 11. 0 nM TMRM. Control measurement in the absence of TMRM. Conditions as in Fig. 1. ∆=0.015 FU. 1
13:57, 11 March 2014 TMRM Fig 10.png (file) 22 KB Figure 10. 0 nM TMRM. Control measurement in the absence of TMRM. Conditions as in Fig. 1. ∆=0.070 FU. 1
13:55, 11 March 2014 TMRM Fig 9.png (file) 22 KB Figure 9. 4000 nM TMRM. MLM incubated with 4000 nM TMRM, other conditions as in Fig. 2, for explanation of changes in mtMP and fluorescence see legend to Fig. 6. ∆=2.535 FU. 1
13:54, 11 March 2014 TMRM Fig 8.png (file) 23 KB Figure 8. 5000 nM TMRM. MLM incubated with 5000 nM TMRM, other conditions as in Fig. 2. , for explanation of changes in mtMP and fluorescence see legend to Fig. 6. The instability of the signal in the presence of ADP may reflect the onset of toxicity at t 1
13:51, 11 March 2014 TMRM Fig 7.png (file) 23 KB Figure 7. 2000 nM TMRM. MLM incubated with 2000 nM TMRM, other conditions as in Fig. 2, for explanation of changes in mtMP and fluorescence see legend to Fig. 6. ∆=0.956 FU. 1
13:50, 11 March 2014 TMRM Fig 6.png (file) 23 KB Figure 6. 1000 nM TMRM. MLM incubated with 1000 nM TMRM, other conditions as in Fig. 2. In the unquenching mode the decrease of mtMP resulting from addition of ADP is mirrored by an increase of fluorescence, while the increase of mtMP due to inhibition of 1
13:49, 11 March 2014 TMRM Fig 5.png (file) 23 KB Figure 5. 500 nM TMRM. MLM incubated with 500 nM TMRM, other conditions as in Fig. 2. At this concentration TMRM is at the verge of alternating between a response in quenching and unquenching mode. In the latter mode the relation between mtMP and TMRM con 1
13:47, 11 March 2014 TMRM Fig 4.png (file) 25 KB Figure 4. 200 nM TMRM. MLM incubated with 200 nM TMRM, other conditions as in Fig. 2. Short-lived peaks of fluorescence observed after addition of chemicals are due to transient unquenching of matrix TMRM caused by the rapid release of the dye. ∆=0.100 1
13:45, 11 March 2014 TMRM Fig 3.png (file) 27 KB Figure 3. 50 nM TMRM. MLM incubated with 50 nM TMRM, other conditions as in Fig. 2. ∆=0.016 FU. 1
13:44, 11 March 2014 TMRM Fig 2.png (file) 26 KB Figure2. 20 nM TMRM. MLM incubated with 20 nM TMRM and energized by CI-linked substrates display a basal level of fluorescence due to accumulation of the dye according to their mtMP in the LEAK state. Upon addition of ADP proton motive force is partially 1
13:40, 11 March 2014 TMRM Fig 1.png (file) 14 KB Figure 1. Zero nM TMRM. Control measurement of MLM in the absence of TMRM showing control respiratory rates (A; blue line: oxygen concentration, red line: oxygen flux) and background fluorescence (B; green line: raw signal of fluorescence, red line: rate 1
09:43, 10 February 2014 Beef heart Mitochondria Malate effect.png (file) 42 KB P:\3C_Projects_Data\2013_all data\2013_Beef heart\2013-11-25 2012-11-25 IJ02 1
14:57, 7 February 2014 Char heart homogenate Malate effect.png (file) 40 KB 2013-10-15 AB-02_A3HL.DLD 1.054 mg/ml, 15°C 1
14:27, 7 February 2014 Char liver homogenate Malate effect.png (file) 42 KB 2013-10-14 AB-03_A2HL.DLD 3.898 mg/ml 15°C 1
13:51, 7 February 2014 Mouse brain homogenate Malate effect.png (file) 44 KB 2013-04-29 MN-02.DLD 2013:Islam\Liver\Mouse brain Mouse brain homogenate 1
13:51, 21 January 2014 Blue Book Fig 4 3.jpg (file) 133 KB   1
10:21, 24 October 2013 Oligomycin-inhibition.jpg (file) 64 KB Image from Peter Schoenfeld ([email protected]) sent in the course of the MiPNet discussion forum on "Problems with oligomycin" 1
10:10, 24 October 2013 Oligomycin-inhibition .jpg (file) 39 KB Image from Peter Schoenfeld ([email protected]) sent in the course of the MiPNet discussion forum on "Problems with oligomycin" 1