Stadlmann 2002 Transplantation

Stadlmann S, Rieger G, Amberger A, Kuznetsov AV, Margreiter R, Gnaiger E (2002) H2O2-mediated oxidative stress versus cold ischemia-reperfusion: mitochondrial respiratory defects in cultured human endothelial cells. Transplantation 74:1800-3.

» PMID: 12499903 Open Access

Stadlmann S, Rieger G, Amberger A, Kuznetsov AV, Margreiter R, Gnaiger Erich (2002) Transplantation

Abstract: Oxidative stress to vascular endothelium plays an important role in cold ischemia-reperfusion (CIR) injury. We compared mitochondrial and plasma membrane integrity in human endothelial cells after 20-min exposure to 500 µM H₂O₂ or 8-hr cold ischemia and simulated reperfusion. In both groups, plasma membrane integrity was maintained but respiration was significantly decreased, as measured by high-resolution respirometry. Uncoupling was more pronounced after H₂O₂ exposure compared with CIR. After H₂O₂ exposure, complex I respiration was significantly reduced, whereas CIR resulted additionally in a significant inhibition of complex II and IV respiration. Our results point to a partial overlap of the patterns of mitochondrial defects after H2O2-mediated and CIR injury. In this respect, H₂O₂ exposure proved to be a useful model to study the mechanisms of CIR injury to human endothelial cells, whereas the full pattern of CIR injury could not be induced by a pulse of hydrogen peroxide exposure. • Keywords: Latent mitochondrial dysfunction

• O2k-Network Lab: AT Innsbruck Oroboros

Cited by

• 8 articles in PubMed (2021-12-27) https://pubmed.ncbi.nlm.nih.gov/12499903/

Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5^th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002

Labels: MiParea: Respiration, mt-Medicine  Pathology: Cardiovascular  Stress:Ischemia-reperfusion, Oxidative stress;RONS  Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell  Preparation: Intact cells, Permeabilized cells 

Regulation: Coupling efficiency;uncoupling, Substrate  Coupling state: LEAK, ROUTINE, OXPHOS  Pathway: N, S, CIV, ROX  HRR: Oxygraph-2k 

Latent mitochondrial dysfunction, BEC 2020.2, MitoFit 2021 PLT