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Stanic 2023 MiPschool Obergurgl

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Stanic 2023 MiPschool Obergurgl

Stanic Sara
Cold-induced non-shivering thermogenesis in skeletal muscle of obesity resistant mice.

Link: MiPschool Obergurgl 2023

Stanic Sara (2023)

Event: MiPschool Obergurgl 2023

Authors: Stanic Sara, Janovska Petra, Zouhar Petr, Bardova K, Otahal J, Vrbacky Marek, Mracek Tomas, Adamcova K, Lenkova L, Funda J, Cajka T, Drahota Zdenek, Rustan Arlid C, Horakova Olga, Houstek Josef, Rosmeissl M, Kopecky Jan

Introduction: Non-shivering thermogenesis (NST) is an energy-dissipating process that occurs in brown adipose tissue (BAT) and is activated by the adrenergic system. Earlier studies found that cold induces adrenergically activated NST in obesity-prone C57BL/6 (B6) mice, but not in obesity-resistant A/J mice. To investigate this difference, we studied the effect of cold acclimation on muscle NST.
Methods: Palmitoyl carnitine oxidation and cytochrome c oxidase (COX) activity (TMPD+ascorbate and KCN) was measured in muscle homogenates of A/J and B6 mice acclimated to 30 ยฐC or to 6 ยฐC using Oroboros Oxygraph. In parallel, amount of mitochondrial supercomplexes was assessed by Blue native electrophoresis.
Results and discussion: As expected, muscle of A/J mice exhibited higher amount of Scaf1 dependent supercomplex III2IV than muscle of B6 mice, and this amount was further increased by cold acclimation. Both palmitoyl carnitine oxidation and COX activity were induced by cold in A/J but not in B6 mice.
The higher oxidation capacity of muscle of cold acclimated A/J mice, possibly connected with supercomplex composition, may indicate that muscle represents the site of alternative NST instead of BAT in these mice. The distinct mechanism of NST could correspond to obesity resistance of this strain.

  1. Janovska P. et al (2023) Impairment of adrenergically-regulated thermogenesis in brown fat of obesity-resistant mice is compensated by non-shivering thermogenesis in skeletal muscle. https://doi.org/10.1016/j.molmet.2023.101683

โ€ข Keywords: Non-shivering thermogenesis, Supercomplexes, cold acclimation


Affiliations and acknowledgements

Stanic Sara1,2, Janovska P1, Zouhar P1, Bardova K1, Otahal J3, Vrbacky M4, Mracek T4, Adamcova K1, Lenkova L1, Funda J1, Cajka T5, Drahota Z4, Rustan AC6, Horakova O1, Houstek J4, Rosmeissl M1, Kopecky J1
  1. Lab. of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
  2. Dept. of Physiology, Faculty of Science, Charles Univ, Prague, Czech Republic
  3. Lab. of Developmental Epileptology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
  4. Lab. of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
  5. Lab. of Translational Metabolism and Laboratory of Bioactive Lipids, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
  6. Section for Pharmacology and Pharmaceutical Biosciences, Dept. of Pharmacy, Univ. of Oslo, Oslo, Norway
Corresponding author: [email protected]
Funding: Output of project no. LX22NPO5104 - funded by the European Union Next Generation EE


Labels:


Organism: Mouse 


Enzyme: Supercomplex 



Event: Poster