Starovlah 2019a MiP2019

From Bioblast
Jump to navigation Jump to search
Isidora Starovlah
Psychophysiological stress changes transcriptional profile of molecular markers of mitochondrial dynamics in myocardium of adult male rats.

Link: MiP2019

Starovlah IM, Radovic SM, Kostic TS, Andric SA (2019)

Event: MiP2019

COST Action MitoEAGLE

Stress is an energy dependent and demanded process and requires the mitochondria as mediators representing the site of crossing the convergent pathways. Although psychophysical stress is the most common stress in human society, the markers of mitochondrial dynamics in myocardium were not described.

The aim of this study was to determine the transcription profile of main markers of mitochondrial dynamics in myocardium of acutely or repeatedly stressed adult male rats. The psychophysiological stress was performed by immobilization (IMO) for 3 hours for one (1xIMO, acute stress) or ten (10xIMO, repeated stress) consecutive days.

RQ-PCR-results revealed that transcription of PGC1, the master regulator of mitochondrial dynamics and integrator of environmental signals, decreased in myocardium from both, acutely and repeatedly stressed rats comparing to undisturbed controls. The transcripts of PGC1-downstream-targets Nrf1/Nrf2 declined only in 10xIMO group. Tfam significantly increased in hearts of both stressed groups. Markers of mitochondrial fusion/architecture, Mfn1 and Opa1, increased in myocardium from 10xIMO group, while Mfn2 remained unchanged. This was followed by reduction of Mtnd1 (mtDNA encoded transcript whose core subunit belongs to the minimal assembly required for catalysis). In the same samples, the transcription of main markers of mitophagy was opposite: Pink increased, but Prkn decreased. Mitochondrial functional markers (Ucp1, Ucp2, Ucp3) increased in myocardium from 10xIMO-group.

According to our best knowledge, these results are the first data showing the transcriptional profile of mitochondrial dynamics markers in myocardium of stressed organism and can be used as a solid base for further multidisciplinary research to clarify the molecular mechanisms.


Bioblast editor: Plangger M, Tindle-Solomon L


Labels: MiParea: mtDNA;mt-genetics, nDNA;cell genetics, Comparative MiP;environmental MiP 


Organism: Rat  Tissue;cell: Heart 





Affiliations and support

Lab Reproductive Endocrinology Signaling, Lab Chronobiology Aging, Centre Excellence CeRES, Fac Sciences, Univ Novi Sad, Novi Sad, Serbia. - [email protected]
This work was supported by the grant no. 173057 and grant no. 451-0302807 Centre of Excellence CeRES from the Ministry of Education, Science and Technological Development, Republic of Serbia, as well as grant no. 2130 from the Autonomous Province of Vojvodina.