Superoxide dismutase

high-resolution terminology - matching measurements at high-resolution

Superoxide dismutase

Description

Mammalian superoxide dismutase (SOD) exists in three forms, of which the Mn-SOD occurs in mitochondria (mtSOD, SOD2; 93 kD homotetramer) and many bacteria, in contrast to the Cu-Zn forms of SOD (cytosolic SOD1, extracellular SOD3 anchored to the extracellular matrix and cell surface). Superoxide anion (O₂^•-) is a major reactive oxygen species (ROS) which is dismutated by SOD to oxygen and H₂O₂.

Abbreviation: SOD

Reference: Fridovich_1997_J Biol Chem

Application in HRR

SOD: Superoxide dismutase from bovine liver, Sigma S8160, 15 KU, store at -20°C; MW = 32.000,00 g/mol

Preparation of 5000 U/mL stock solution (dissolved in H₂O)

Please check the concentration of the batch to calculate the volume needed to obtain a stock solution of 5000 U/mL using 2 mg of superoxide dismutase.

1. Weigh 2 mg of superoxide dismutase.
2. Calculate the volume needed to obtain a stock solution of 5000 U/mL (e.g. 1134 µL H₂O for a batch with a concentration of 2835 U/mg).
3. Dissolve.
4. Divide into 30 µL portions into 0.2 mL Eppendorf tubes.
5. Store frozen at -20 °C in the desiccator.

• Add SOD for conversion of O₂^•- and Measurement of H₂O₂ with Amplex UltraRed.

MitoPedia topics: Enzyme