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TPP special interest group during an International Oxygraph Course

From Bioblast

Oroboros Instruments offers special interest groups during its International Oxygraph Courses (IOC) covering the utilization of the Oroboros ISE system to measure mitochondrial membrane potential. Some topics of general interest will be covered together with the participants from the "main course". This special interest groups is intended as a workshop, therefor the exact program will depend on the level of experience and interests of the participants. Typical topics covered will include:


  • Introduction to measurement of mitochondrial membrane potential with a TPP-electrode.
  • Planning and performing a TPP Experiment: Introduction.
  • Hands on: Assembly and maintenance of TPP and reference electrodes; Set up of the instrument with TPP and reference electrodes.
  • Hands-on: Instrumental background oxygen flux in the presence of the TPP electrode.
  • Hands-on: TPP calibration and TPP chemical background.
  • From the TPP+ signal to mitochondrial membrane potential - Guide through the Excel templates.
  • Hands-on: Exercise with DatLab demo files and Discussion
  • Circumstances and time frame permitting: Hands-on: Extending the TPP method for the determination of mitochondrial membrane potential beyond isolated mitochondria: TPP calibration and experiment with biological sample.


MultiSensor applications: