Talk:ADP

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Former Instructions

These preparation instructions are still correct. Ordering ADP in portions of 1 g per vial turned out to be very useful and is therefore the recommended strategy (as described on the ADP main page).

Preparation without MgCl2 and lower amounts of ADP

Preparation of 500 mM ADP stock solution (dissolved in H2O):
  1. Weigh 501.3 mg of ADP = 1 mmol ADP
  2. Add 1.2 mL H2O (ADP is not dissolved at this stage).
  3. Neutralize with 5 M KOH (approx. 450 µL). ADP will dissolve after addition of KOH.
  4. Check pH and adjust to 7 with 5 M KOH if necessary.
  5. Adjust final volume to 2 mL and divide into 0.2 mL portions.
  6. Store at -80 °C.

Preparation with MgCl2 and lower amounts of ADP

Preparation of 500 mM ADP stock solution with 300 mM free Mg2+ (dissolved in H2O):
  1. Weigh 501.3 mg of ADP = 1 mmol ADP.
  2. Add 1.2 mL H2O (ADP is not dissolved at this stage).
  3. Neutralize with 5 M KOH (approx. 450 µl). ADP will dissolve after addition of KOH.
  4. Add 121.98 mg MgCl2. White precipitate will occur, which will dissolve during 1-2 min stirring on magnetic stirrer at RT.
  5. Check pH and adjust to 7 with 5 M KOH if necessary.
  6. Adjust final volume to 2 mL and divide into 0.2 mL portions.
  7. Store at -80 °C.