Tallosy 2021 Sci Rep

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Tallósy SP, Poles MZ, Rutai A, Fejes R, Juhász L, Burián K, Sóki J, Szabó A, Boros M, Kaszaki J (2021) The microbial composition of the initial insult can predict the prognosis of experimental sepsis. Sci Rep 11:22772.

» PMID: 34815465 Open Access

Tallosy Szabolcs Peter, Poles Marietta Zita, Rutai Attila, Fejes Roland, Juhasz Laszlo, Burian Katalin, Soki Jozsef, Szabo Andrea, Boros Mihaly, Kaszaki Jozsef (2021) Sci Rep

Abstract: We hypothesized that the composition of sepsis-inducing bacterial flora influences the course of fecal peritonitis in rodents. Saline or fecal suspensions with a standardized dose range of bacterial colony-forming units (CFUs) were injected intraperitoneally into Sprague-Dawley rats. The qualitative composition of the initial inoculum and the ascites was analyzed separately by MALDI-TOF mass spectrometry. Invasive monitoring was conducted in separate anesthetized groups (n = 12-13/group) after 12, 24, 48 and 72 h to determine rat-specific organ failure assessment (ROFA) scores. Death and ROFA scores peaked at 24 h. At this time, 20% mortality occurred in animals receiving a monomicrobial E. coli suspension, and ROFA scores were significantly higher in the monomicrobial subgroup than in the polymicrobial one (median 6.5; 5.0-7.0 and 5.0; 4.75-5.0, respectively). ROFA scores dropped after 48 h, accompanied by a steady decrease in ascites CFUs and a shift towards intra-abdominal monomicrobial E. coli cultures. Furthermore, we found a relationship between ascites CFUs and the evolving change in ROFA scores throughout the study. Hence, quantitatively identical bacterial loads with mono- or polymicrobial dominance lead to a different degree of sepsis severity and divergent outcomes. Initial and intraperitoneal microbiological testing should be used to improve translational research success.

Bioblast editor: Plangger M O2k-Network Lab: HU Szeged Boros M


Labels: MiParea: Respiration  Pathology: Sepsis 

Organism: Rat  Tissue;cell: Liver  Preparation: Homogenate 


Coupling state: LEAK, OXPHOS  Pathway: S, ROX  HRR: Oxygraph-2k 

2021-12