Template:Chemicals SUIT-006 Q mt D071
From Bioblast
| Step | Chemical(s) and link(s) | Comments |
|---|---|---|
| 1Rot | Rotenone (Rot) | This step is essential to reach the fully oxidized CoQ2 needed to calculate the reduced Q fraction. |
| 1S | Succinate (S) | |
| 2D | ADP (D) | |
| (3Omy) | Oligomycin (Omy) | This step can be skipped. |
| 4U | Carbonyl cyanide m-chlorophenyl hydrazone, CCCP (U) | Can be substituted for other uncoupler. |
| 5Anox | The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration. | |
| 6Ama | Antimycin A (Ama) | This step can be omitted. |
