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Template:SUIT-007 pce

From Bioblast
MitoPedia: SUIT

Steps and respiratory states

Ce1;1Dig;1G;2D;2c;3M;4U;5Ama.png

Step State Pathway Q-junction Comment - Events (E) and Marks (M)
ce1 ROUTINE ce1
  • ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
1Dig REN ce1;1Dig
  • Optimum effective digitonin concentration for complete plasma membrane permeabilization.
Step State Pathway Q-junction Comment - Events (E) and Marks (M)
1G GL (N) CI 1G
2D GP (N) CI 1G;2D
2c GcP (N) CI 1G;2D;2c
  • Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]).
  • OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
3M GMP N CI 1G;2D;2c;3M
4U GME N CI 1G;2D;2c;3M;4U
5Ama ROX 1G;2D;2c;3M;4U;5Ama
  • Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).


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