Template:SUIT text D007
From Bioblast
The SUIT-002 O2 ce-pce D007 protocol in combination with SUIT-001 O2 ce-pce D003 provides a common reference for comparison of respiratory control of permeabilized cells (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol) in a wide variety of cell types. SUIT-002 O2 ce-pce D007 is specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways. Moreover, the pathway control in OXPHOS state (F, F(N), FN, FNS, FNSGp pathways) and in ET state (FNSGp and SGp) can be evaluated by using this SUIT protocol. SUIT-002 O2 ce-pce D007 can be extended with the CIV assay module.
