Thomas 2011 Mitochondrion

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Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP Jr (2011) Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11:108-18.

» PMID: 20727424 Open Access

Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP (2011) Mitochondrion

Abstract: Recombinant human mitochondrial transcription factor A protein (rhTFAM) was evaluated for its acute effects on cultured cells and chronic effects in mice. Fibroblasts incubated with rhTFAM acutely increased respiration in a chloramphenicol-sensitive manner. SH-SY5Y cells showed rhTFAM concentration-dependent reduction of methylpyridinium (MPP+)-induced oxidative stress and increases in lowered ATP levels and viability. Mice treated with weekly i.v. rhTFAM showed increased mitochondrial gene copy number, Complex I protein levels and ATP production rates; oxidative damage to proteins was decreased ~50%. rhTFAM treatment improved motor recovery rate after treatment with MPTP and dose-dependently improved survival in the lipopolysaccharide model of endotoxin sepsis.

Keywords: Recombinant TFAM, Mitochondrial biogenesis, ATP synthesis, MPTP, Sepsis

O2k-Network Lab: US VA Richmond Bennett JP, US VA Richmond Iyer S


Stress:Oxidative stress;RONS  Organism: Human, Mouse  Tissue;cell: Heart, Skeletal muscle, Nervous system, Fibroblast  Preparation: Homogenate, Isolated mitochondria 

Regulation: ATP, ATP production 

HRR: Oxygraph-2k