Timon-Gomez 2014 Abstract MiP2014

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The function of mitochondrial pyruvate carriers in the adaptation of respiration capacity and stress tolerance in yeast.
Link:
Timon-Gomez A
Mitochondr Physiol Network 19.13 - MiP2014

Timon-Gomez A, Proft M, Pascual-Ahuir A (2014)

Event: MiP2014

Yeast cells adapt mitochondrial morphology, biomass and activity to changing environmental conditions in a dynamic manner. Here we investigate the mechanisms of mitochondrial adaptation to different respiration rates, oxidative and salt stress.

Specifically, we identifed the MPC gene family as particularly regulated upon changes from fermentative to respiratory growth or upon stress. Mpc proteins are highly conserved from yeast to humans and are necessary for the uptake of pyruvate into mitochondria [1,2], which is used for leucine and valine biosynthesis and as a fuel for respiration.

The highly similar Mpc2 and Mpc3 proteins are regulated in an antagonistic manner: Mpc2 is most abundant under fermentative non-stress conditions and important for amino acid biosynthesis, while Mpc3 is most abundant upon salt stress or respiratory growth. Overexpression experiments demonstrate that Mpc3 stimulates respiration and oxidative stress tolerance, while Mpc2 inhibits respiration and oxidative stress tolerance. Therefore, the regulated mitochondrial pyruvate uptake via different Mpc proteins might be an important determinant of respiration rate and stress resistance [3]. We additionally analyzed the degradation rate of different respiratory complex subunits in response to higher respiration rates and mitochondrial damage.


Labels: MiParea: Respiration, Comparative MiP;environmental MiP 

Stress:Oxidative stress;RONS  Organism: Saccharomyces cerevisiae 

Preparation: Intact organism 



Event: B3, P-flash  MiP2014: salt stress 

Affiliation

Inst Biol Mol Celular Plantas, CSIC-Univ Politécnica Valencia, Spain. - altimgme@etsia.upv.es

References

  1. Bricker DK, Taylor EB, Schell JC, Orsak T, Boutron A, Chen YC, Cox JE, Cardon CM, Van Vranken JG, Dephoure N, Redin C, Boudina S, Gygi SP, Brivet M, Thummel CS, Rutter J (2012) A mitochondrial pyruvate carrier required for pyruvate uptake in yeast, Drosophila, and humans. Science 337: 96-100.
  2. Herzig S, Raemy E, Montessuit S, Veuthey JL, Zamboni N, et al (2012) Identification and functional expression of the mitochondrial pyruvate carrier. Science 337: 93-6.
  3. Timón-Gómez A, Proft M, Pascual-Ahuir A (2013) Differential regulation of mitochondrial pyruvate carrier genes modulates respiratory capacity and stress tolerance in yeast. PloS One 8: e79405.