Tomec 1975 Arch Biochem Biophys

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Tomec RJ, Hoppel CL (1975) Carnitine palmitoyltransferase in bovine fetal heart mitochondria. Arch Biochem Biophys 170:716-23.

» PMID:1190787

Tomec RJ, Hoppel CL (1975) Arch Biochem Biophys

Abstract: Bovine fetal heart mitochondria oxidize palmitoyl-CoA in the presence of I-carnitine at 2-148 of the rate they oxidize palmitoyl-I-camitine when incubation times are less than 5 min. The initial low oxidation rate with palmitoyl-CoA is not significantly changed by increasing the concentration of ADP in the incubation. Preincubation of fetal heart mitochondria with palmitofl-CoA plus carnitine for 10 min before initiating oxidation with ADP leads to oxidation rates that are comparable to those obtained in calf heart mitochondria. The total camitine palmitoyltransferase activity in fetal heart mitochondria is not strikingly different from calf heart mitochondria. The palmitoyl- CoA saturation curves show that deficiency in “overt” carnitine palmitoyltransferase(A) activity in fetal heart mitochondria cannot account for these observations. Our results show an abnormal CoA saturation curve of fetal heart mitochondrial transferase activity but the relationship of this finding to the lag period before maximal oxidation of palmitoyl-CoA is not known.

Cited by

  • Komlodi et al (2021) Hydrogen peroxide production, mitochondrial membrane potential and the redox state of the coenzyme Q measured at tissue normoxia and experimental hyperoxia in heart mitochondria. MitoFit Preprints 2021 (in prep)

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MitoFit 2021 Tissue normoxia