Uribe-Alvarez 2016 Abstract MitoFit Science Camp 2016
|In Staphylococcus epidermidis, oxygen variations promote differential expression of respiratory enzymes that constitute possible therapeutic targets.|
Staphylcoccus epidermidis does not invade healthy tissues, however, it has been identified as a cause of nosocomial infections due to its ability to form biofilms on polymer surfaces . S. epidermidis can be grown at different oxygen concentrations ([O2]), including mammalian skin where [O2] ranges from 3-5% and in anaerobic altered tissues [2,3].
Biofilm formation of S. epidermidis and its respiratory chain components grown in aerobic, microaerobic and anaerobic conditions were evaluated by in-gel activities, enzymatic activities, spectrophotometry and oxymetry. Varying [O2] modified both biofilm formation and the components in the respiratory chain: At high [O2], little tendency to form biofilms was observed. S. epidermidis expressed glycerol-3-phosphate, pyruvate, ethanol and succinate dehydrogenases; and cyt bo and aa3. Under micro-aerobiosis, biofilm formation increased slightly; pyruvate, ethanol, glycerol-3-phosphate and succinate dehydrogenase decreased; aa3 cyt was not detected; Under anaerobiosis high biofilm-formation and low ethanol and pyruvate dehydrogenase activities were found; anaerobic nitrate dehydrogenase activity was detected. Aerobic-grown cells with cyanide increased biofilm formation. Anaerobic-grown cells with methylamine decreased biofilm formation.
Thus, either a decrease in [O2] or the inhibition of the aerobic chain led S. epidermidis to associate into biofilms. In contrast, high [O2] or inhibition of the anaerobic nitrate reductase prevented biofilm formation suggesting that the enzymes expressed at low to null [O2] are therapeutic targets against biofilm formation by S. epidermidis.
• O2k-Network Lab: MX Mexico City Uribe-Carvajal S
Regulation: Oxygen kinetics
HRR: Oxygraph-2k Event: B2 MitoFit Science Camp 2016
1-Dept Mol Genet, IFC, UNAM, Mexico; 2-Dept Cell Developm Biol, IFC, UNAM, Mexico, 3-Nijmegen Center Mitochondrial Disorders, Radboud Univ Med Center, The Netherlands. - [email protected]
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