Uribe-Alvarez 2016 Abstract MitoFit Science Camp 2016

From Bioblast
Jump to navigation Jump to search
In Staphylococcus epidermidis, oxygen variations promote differential expression of respiratory enzymes that constitute possible therapeutic targets.

Link:

Cristina Uribe-Alvarez

Uribe-Alvarez C, Chiquete-Felix N, Contreras-Zentella M, Guerrero-Castillo S, Pena A, Uribe-Carvajal S (2016)

Event: MitoFit Science Camp 2016 Kuehtai AT

Staphylcoccus epidermidis does not invade healthy tissues, however, it has been identified as a cause of nosocomial infections due to its ability to form biofilms on polymer surfaces [1]. S. epidermidis can be grown at different oxygen concentrations ([O2]), including mammalian skin where [O2] ranges from 3-5% and in anaerobic altered tissues [2,3].

Biofilm formation of S. epidermidis and its respiratory chain components grown in aerobic, microaerobic and anaerobic conditions were evaluated by in-gel activities, enzymatic activities, spectrophotometry and oxymetry. Varying [O2] modified both biofilm formation and the components in the respiratory chain: At high [O2], little tendency to form biofilms was observed. S. epidermidis expressed glycerol-3-phosphate, pyruvate, ethanol and succinate dehydrogenases; and cyt bo and aa3. Under micro-aerobiosis, biofilm formation increased slightly; pyruvate, ethanol, glycerol-3-phosphate and succinate dehydrogenase decreased; aa3 cyt was not detected; Under anaerobiosis high biofilm-formation and low ethanol and pyruvate dehydrogenase activities were found; anaerobic nitrate dehydrogenase activity was detected. Aerobic-grown cells with cyanide increased biofilm formation. Anaerobic-grown cells with methylamine decreased biofilm formation.

Thus, either a decrease in [O2] or the inhibition of the aerobic chain led S. epidermidis to associate into biofilms. In contrast, high [O2] or inhibition of the anaerobic nitrate reductase prevented biofilm formation suggesting that the enzymes expressed at low to null [O2] are therapeutic targets against biofilm formation by S. epidermidis.


O2k-Network Lab: MX Mexico City Uribe-Carvajal S


Labels:


Organism: Eubacteria 


Regulation: Oxygen kinetics 


HRR: Oxygraph-2k  Event: B2  MitoFit Science Camp 2016 

Affiliations

1-Dept Mol Genet, IFC, UNAM, Mexico; 2-Dept Cell Developm Biol, IFC, UNAM, Mexico, 3-Nijmegen Center Mitochondrial Disorders, Radboud Univ Med Center, The Netherlands. - [email protected]

References

  1. Otto M (2009) Staphylococcus epidermidis-the 'accidental' pathogen. Nat Rev Microbiol 7:555-67.
  2. Peyssonnaux C, Boutin AT, Zinkernagel AS, Datta V, Nizet V, Johnson RS (2008) Critical role of HIF-1alpha in keratinocyte defense against bacterial infection. J Invest Dermatol 128:1964-8.
  3. Wiese M, Gerlach RG, Popp I, Matuszak J, Mahapatro M, Castiglione K, Chakravortty D, Willam C, Hensel M, Bogdan C, Jantsch J (2012) Hypoxia-mediated impairment of the mitochondrial respiratory chain inhibits the bactericidal activity of macrophages. Infect Immun 80:1455-66.