Wenchich 2003 Physiol Res

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Wenchich L, Drahota Z, Honzík T, Hansíková H, Tesarová M, Zeman J, Houstek J (2003) Polarographic evaluation of mitochondrial enzymes activity in isolated mitochondria and in permeabilized human muscle cells with inherited mitochondrial defects. Physiol Res 52:781-88.

» PMID: 14640901 Open Access

Wenchich L, Drahota Z, Honzik T, Hansikova H, Tesarova M, Zeman J, Houstek J (2003) Physiol Res

Abstract: Inherited disturbances of the mitochondrial energy generating system represent a heterogeneous group of disorders associated with a broad spectrum of metabolic abnormalities and clinical symptoms. We used the polarographic and spectrophotometric method for detection of mitochondrial disorders, because these two techniques provide a different insight into mitochondrial function. In six patients suspected of mitochondrial disease we found defects of complex I (two patients), complex III (one patient), complex IV (two patients) and a combination of defect of complex III and IV (one patient). Citrate synthase activity, used as the reference enzyme, was not changed. A comparison of the two methods showed several differences in evaluation of mitochondrial enzymes activity due to the fact that both methods used different conditions for enzyme activity measurements. In contrast to oxygen consumption measurements, where the function of the whole-integrated respiratory chain is characterized, spectrophotometric measurements characterize activities of isolated complexes in disintegrated membranes. However, it may be concluded from our experiments that both methods provide useful and complementary data about mitochondrial energetic functions. Whereas spectrophotometric data are suitable for evaluation of maximal enzyme activities of mitochondrial enzyme complexes, polarographic data provide better information about enzyme activities in cells with mitochondrial defects under in situ conditions.

Keywords: Mitochondria, Muscle cells, Respiratory chain complexes, Polarography, Spectrophotometry

O2k-Network Lab: CZ Prague Zeman J, CZ Prague Houstek J, CZ Hradec Kralove Cervinkova Z


Labels:

Stress:Mitochondrial disease  Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue, Isolated mitochondria 



HRR: Oxygraph-2k 

Spectrophotometry; Spectrofluorimetry