Wieckowski 2014 Abstract MiP2014

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Understanding the utility and limitations of autopsy samples for mitochondrial studies - evaluation of oxidative stress in the central nervous system of patients with diagnosed or highly probable mitochondrial diseases.
Link:
Wieckowski MR
Mitochondr Physiol Network 19.13 - MiP2014

Karkucinska-Wieckowska A, Suski JM, Wojtala A, Pronicki M, Duszynski J, Wieckowski MR (2014)

Event: MiP2014

Genetic mitochondrial diseases occur as a result of mutations in the nuclear or mitochondrial genome. Such impairment is often associated with dysfunctions in proteins which are part of the respiratory system. First, our aim was to study whether the time elapsed from death until dissection has a significant impact on the detected protein damage and thus affects or distorts the results.

To address this topic, brain samples were obtained from mice. After sacrificing the animals, they were stored under the same conditions as the remains of patients, and dissected in a similar time regime as when obtaining autopsy samples from deceased patients.

Our results demonstrated no significant alterations in protein levels and damage analyzed at successive time points. Our results suggest that autopsy samples can be used for the study of oxidative damage, which can extend and direct further research toward the diagnosis of mitochondrial diseases, which are accompanied by elevated levels of reactive oxygen species.


Labels: MiParea: mt-Medicine 

Stress:Oxidative stress;RONS  Organism: Mouse  Tissue;cell: Nervous system 




Event: C4, P-flash  MiP2014 

Affiliation

1-Dep Biochem, Nencki Inst Experim Biol; 2-Dep Pathology, Children’s Mem Health Inst; Warsaw, Poland. - m.wieckowski@nencki.gov.pl

Acknowledgements

Supported by the Internal Projects of CMHI 125/2012, Statutory Founding from Nencki Institute of Experimental Biology, Polish Ministry of Science, Higher Education grant W100/HFSC/2011 and a grant from the Polish National Science Centre (UMO-2011/01/M/NZ3/02128).