Yang 2017 Cell Death Dis

From Bioblast
Publications in the MiPMap
Yang N, Weinfeld M, Lemieux H, Montpetit B, Goping IS (2017) Photo-activation of the delocalized lipophilic cation D112 potentiates cancer selective ROS production and apoptosis. Cell Death Dis 8:e2587.

Β» PMID: 28151485 Open Access

Yang N, Weinfeld M, Lemieux H, Montpetit B, Goping IS (2017) Cell Death Dis

Abstract: Delocalized lipophilic cations (DLCs) selectively accumulate in cancer cell mitochondria and have long been explored for therapeutic applications. Although targeted effects to cancer cells are demonstrated in vitro, non-specific toxicities in vivo have hampered clinical development. Identifying the molecular mechanisms of action and enhancing selectivity are thus necessary next steps to improve these compounds and evaluate their suitability for further drug development. D112 is one such DLC with promising properties. We previously demonstrated that D112 selectively induced intrinsic apoptosis in transformed versus non-transformed cell lines. Here we show that D112 preferentially entered transformed cells where it interacted with, and damaged mitochondrial DNA, inhibited Complex I respiration and induced reactive oxygen species (ROS). ROS production was critical for Bax activation and subsequent apoptosis. Importantly, photo-activation of D112 potentiated selective ROS production and increased the window of toxicity towards cancer cells over non-transformed cells. Thus photodynamic therapy would be an exciting adjunct to D112 studies and may be generally applicable for other DLCs that are currently under therapeutic investigation. β€’ Keywords: Human Jurkat Jneo cells β€’ Bioblast editor: Kandolf G β€’ O2k-Network Lab: CA Edmonton Lemieux H

Labels: MiParea: Respiration, mtDNA;mt-genetics, mt-Medicine, Pharmacology;toxicology  Pathology: Cancer  Stress:Cell death  Organism: Human, Saccharomyces cerevisiae  Tissue;cell: Lymphocyte  Preparation: Intact cells, Permeabilized cells 

Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: N, S, CIV, NS, ROX  HRR: Oxygraph-2k 

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