Zweck 2023 ESC Heart Fail

From Bioblast
Publications in the MiPMap
Zweck E, Karschnia M, Scheiber D, Heidecke H, Dechend R, Barthuber C, Kaufmann S, Kelm M, Roden M, Westenfeld R, SzendrΓΆdi J, Boege F (2023) Receptor autoantibodies: Associations with cardiac markers, histology, and function in human non-ischaemic heart failure.

Β» ESC Heart Fail 10:1258-69. PMID: 36717981 Open Access

Zweck Elric, Karschnia Maximilian, Scheiber Daniel, Heidecke Harald, Dechend Ralf, Barthuber Carmen, Kaufmann Sina, Kelm Malte, Roden Michael, Westenfeld Ralf, Szendroedi Julia, Boege Fritz (2023) ESC Heart Fail

Abstract: A causal link between non-ischaemic heart failure (HF) and humoral autoimmunity against G-protein-coupled receptors (GPCR) remains unclear except for Chagas' cardiomyopathy. Uncertainty arises from ambiguous reports on incidences of GPCR autoantibodies, spurious correlations of autoantibody levels with disease activity, and lack of standardization and validation of measuring procedures for putatively cardio-pathogenic GPCR autoantibodies. Here, we use validated and certified immune assays presenting native receptors as binding targets. We compared candidate GPCR autoantibody species between HF patients and healthy controls and tested associations of serum autoantibody levels with serological, haemodynamic, metabolic, and functional parameters in HF.

Ninety-five non-ischaemic HF patients undergoing transcatheter endomyocardial biopsy and 60 healthy controls were included. GPCR autoantibodies were determined in serum by IgG binding to native receptors or a cyclic peptide (for Ξ²1AR autoantibodies). In patients, cardiac function, volumes, and myocardial structural properties were assessed by cardiac magnetic resonance imaging; right heart catheterization served for determination of cardiac haemodynamics; endomyocardial biopsies were used for histological assessment of cardiomyopathy and determination of cardiac mitochondrial oxidative function by high-resolution respirometry.

Autoantibodies against Ξ²1 adrenergic (Ξ²1AR) , M5-muscarinic (M5AR), and angiotensin II type 2 receptors (AT2R) were increased in HF (all P < 0.001). Autoantibodies against Ξ±1 -adrenergic (Ξ±1 AR) and angiotensin II type 1 receptors (AT1R) were decreased in HF (all P < 0.001). Correlation of alterations of GPCR autoantibodies with markers of cardiac or systemic inflammation or cardiac damage, haemodynamics, myocardial histology, or left ventricular inflammation (judged by T2 mapping) were weak, even when corrected for total IgG. Ξ²1 AR autoantibodies were related inversely to markers of left ventricular fibrosis indicated by T1 mapping (r = -0.362, P < 0.05) and global longitudinal strain (r = -0.323, P < 0.05). AT2R autoantibodies were associated with improved myocardial mitochondrial coupling as measured by high-resolution respirometry in myocardial biopsies (r = -0.352, P < 0.05). In insulin-resistant HF patients, AT2R autoantibodies were decreased (r = -.240, P < 0.05), and AT1R autoantibodies were increased (r = 0.212, P < 0.05).

GPCR autoantibodies are markedly altered in HF. However, they are correlated poorly or even inversely to haemodynamic, metabolic, and functional markers of disease severity, myocardial histology, and myocardial mitochondrial efficiency. These observations do not hint towards a specific cardio-pathogenic role of GPCR autoantibodies and suggest that further investigations are required before specific therapies directed at GPCR autoantibodies can be clinically tested in non-ischaemic HF. β€’ Keywords: Autoimmunity, Chronic non-ischaemic heart failure, Heart failure, Pathophysiology β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: DE Duesseldorf Roden M, DE Duesseldorf Westenfeld R

Labels: MiParea: Respiration  Pathology: Cardiovascular 

HRR: Oxygraph-2k 


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