SUIT-002 O2 mt D005: Difference between revisions
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== Harmonized SUIT protocols == | == Harmonized SUIT protocols == | ||
=== RP1 and RP2 === | |||
'''SUIT RP1''' [[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1]] Β | '''SUIT RP1''' [[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1]] Β | ||
'''SUIT RP2''' [[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|500px|SUIT-RP2]] Β | '''SUIT RP2''' [[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|500px|SUIT-RP2]] Β | ||
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[[File:RP1&RP2.png|400px]] '''Harmonization between RP1 and RP2''' | [[File:RP1&RP2.png|400px]] '''Harmonization between RP1 and RP2''' | ||
=== RP1 and RP2 in cells === | |||
RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Electron transfer-pathway state|Electron transfer-pathway statea]]. | RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Electron transfer-pathway state|Electron transfer-pathway statea]]. | ||
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=== RP1 and RP2 in PBMCs and PTLs === | |||
In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: [[1PM;2D;3U;4G;5S;6Rot;7Gp-|SUIT 1 for PBMCs and PTLs]] | |||
:::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#Harmonized_SUIT_protocols|SUIT RP1 in PBMCs and PTLs]]'''SUIT RP1 for PBMCs and PLTs''':1PM;2D;2c;3U;4G;5S;6Rot;'''7Gp''';8Ama;'''9AsTm''';10Azd | |||
Β | :::: '''SUIT RP2 PBMCs and PLTs''': 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd | ||
:::: [[ | |||
Β | |||
Β | |||
[[File:Reference protocol RP1&RP2_pce_PBMCs_PTLs.png|400px]] '''Harmonization between RP1 and RP2 in PBMC and PTL cells''' | |||
== SUIT O2 DLP for SUIT 1 (RP1) (DatLab 7.3) == | |||
''Updating this page (2018-06-13) for DatLab 7.3 DLP files'' | |||
Revision as of 14:06, 13 June 2018
Description
Abbreviation: FNSGp(PGM)
Reference: A: SUIT 2 is the RP2 - MiPNet21.06 SUIT RP
SUIT number: 2
MitoPedia concepts:
SUIT protocol,
SUIT A
- MitoPedia: SUIT - SUIT reference protocol RP2
- SUIT-category: FNSGp(PGM)
- SUIT protocol pattern: diametral
Harmonized SUIT protocols
RP1 and RP2
- SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd
- SUIT RP2: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2
RP1 and RP2 in cells
RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet Electron transfer-pathway statea.
- SUIT RP1 in cells: ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2 in cells: ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd
Harmonization between RP1 and RP2 in cells
RP1 and RP2 in PBMCs and PTLs
In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: SUIT 1 for PBMCs and PTLs
- SUIT RP1 in PBMCs and PTLsSUIT RP1 for PBMCs and PLTs:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd
- SUIT RP2 PBMCs and PLTs: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2 in PBMC and PTL cells
SUIT O2 DLP for SUIT 1 (RP1) (DatLab 7.3)
Updating this page (2018-06-13) for DatLab 7.3 DLP files