Majiene 2019 Nutrients: Difference between revisions
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Cell viability was assessed spectrophotometrically using metabolic activity method, and by fluorescent Hoechst/propidium iodide nuclear staining. ROS generation was measured fluorometrically using DCFH-DA. Oxygen uptake rates were recorded by the high-resolution respirometer Oxygraph-2k. | Cell viability was assessed spectrophotometrically using metabolic activity method, and by fluorescent Hoechst/propidium iodide nuclear staining. ROS generation was measured fluorometrically using DCFH-DA. Oxygen uptake rates were recorded by the high-resolution respirometer Oxygraph-2k. | ||
Plumbagin and menadione displayed highly cytotoxic activity on C6 cells ( | Plumbagin and menadione displayed highly cytotoxic activity on C6 cells (IC<sub>50</sub> is 7.7 ยฑ 0.28 ฮผM and 9.6 ยฑ 0.75 ฮผM, respectively) and caused cell death by necrosis. Additionally, they increased the amount of intracellular ROS in a concentration-dependent manner. Moreover, even at very small concentrations (1-3 ยตM), these compounds significantly uncoupled mitochondrial oxidation from phosphorylation impairing energy production in cells. Lawsone had significantly lower viability decreasing and mitochondria-uncoupling effect, and exerted strong antioxidant activity. | ||
Plumbagin and menadione exhibit strong prooxidant, mitochondrial oxidative phosphorylation uncoupling and cytotoxic activity. In contrast, lawsone demonstrates a moderate effect on C6 cell viability and mitochondrial functions, and possesses strong antioxidant properties. | Plumbagin and menadione exhibit strong prooxidant, mitochondrial oxidative phosphorylation uncoupling and cytotoxic activity. In contrast, lawsone demonstrates a moderate effect on C6 cell viability and mitochondrial functions, and possesses strong antioxidant properties. | ||
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{{Labeling | {{Labeling | ||
|area=Respiration, Pharmacology;toxicology | |area=Respiration, Comparative MiP;environmental MiP, Pharmacology;toxicology | ||
|diseases=Cancer | |diseases=Cancer | ||
|organism=Rat | |||
|tissues=Nervous system | |||
|preparations=Permeabilized cells | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=N, NS | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=Labels, 2019-08, | |additional=Labels, 2019-08, | ||
}} | }} |
Latest revision as of 15:12, 12 August 2019
Majiene D, Kuseliauskyte J, Stimbirys A, Jekabsone A (2019) Comparison of the effect of native 1,4-naphthoquinones plumbagin, menadione, and lawsone on viability, redox status, and mitochondrial functions of C6 glioblastoma cells. Nutrients 11:E1294. |
Majiene D, Kuseliauskyte J, Stimbirys A, Jekabsone A (2019) Nutrients
Abstract: 1,4-naphthoquinones, especially juglone, are known for their anticancer activity. However, plumbagin, lawsone, and menadione have been less investigated for these properties. Therefore, we aimed to determine the effects of plumbagin, lawsone, and menadione on C6 glioblastoma cell viability, ROS production, and mitochondrial function.
Cell viability was assessed spectrophotometrically using metabolic activity method, and by fluorescent Hoechst/propidium iodide nuclear staining. ROS generation was measured fluorometrically using DCFH-DA. Oxygen uptake rates were recorded by the high-resolution respirometer Oxygraph-2k.
Plumbagin and menadione displayed highly cytotoxic activity on C6 cells (IC50 is 7.7 ยฑ 0.28 ฮผM and 9.6 ยฑ 0.75 ฮผM, respectively) and caused cell death by necrosis. Additionally, they increased the amount of intracellular ROS in a concentration-dependent manner. Moreover, even at very small concentrations (1-3 ยตM), these compounds significantly uncoupled mitochondrial oxidation from phosphorylation impairing energy production in cells. Lawsone had significantly lower viability decreasing and mitochondria-uncoupling effect, and exerted strong antioxidant activity.
Plumbagin and menadione exhibit strong prooxidant, mitochondrial oxidative phosphorylation uncoupling and cytotoxic activity. In contrast, lawsone demonstrates a moderate effect on C6 cell viability and mitochondrial functions, and possesses strong antioxidant properties. โข Keywords: C6 glioma cell culture, Lawsone, Menadione, Mitochondrial respiration, Plumbagin, Reactive oxygen species โข Bioblast editor: Plangger M
Labels: MiParea: Respiration, Comparative MiP;environmental MiP, Pharmacology;toxicology
Pathology: Cancer
Organism: Rat Tissue;cell: Nervous system Preparation: Permeabilized cells
Coupling state: LEAK, OXPHOS, ET
Pathway: N, NS
HRR: Oxygraph-2k
Labels, 2019-08