Cooper 2000 Sunderland (MA): Sinauer Associates

Cooper GM (2000) The Cell: A Molecular Approach. 2nd edition. Sunderland (MA): Sinauer Associates Available from: https://www.ncbi.nlm.nih.gov/books/NBK9885/

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Cooper GM (2000) Sunderland (MA): Sinauer Associates

Abstract: As in the first edition, The Cell is focused on the molecular biology of cells as a unifying theme, with specialized topics discussed throughout the book as examples of more general principles. Aspects of developmental biology, the immune system, the nervous system, and plant biology are thus discussed in their broader biological context in chapters covering areas such as genome structure, gene expression, DNA rearrangements, the plasma membrane, cell signaling, and the cell cycle. Relationships between cell biology and medicine are similarly discussed throughout the text, as well as being highlighted in the Molecular Medicine essays that are included as a special feature in each chapter. These discussions illustrate the striking impact of molecular and cellular biology on human health, and are intended to stimulate as well as inform those students interested in medicine.

• Bioblast editor: Gnaiger E

Complex II ambiguities - selected qotes from Chapter 10

• Complex II ambiguities

(1) 'Electrons from NADH enter the electron transport chain in complex I, .. A distinct protein complex (complex II), which consists of four polypeptides, receives electrons from the citric acid cycle intermediate, succinate (Figure 10.9). These electrons are transferred to FADH₂, rather than to NADH, and then to coenzyme Q.'

Comment: Here, the frequent comparison is made between FADH₂ (linked to CII) and NADH (linked to CI).

(2) 'In contrast to the transfer of electrons from NADH to coenzyme Q at complex I, the transfer of electrons from FADH₂ to coenzyme Q is not associated with a significant decrease in free energy and, therefore, is not coupled to ATP synthesis.'

Comment: Note that CI is in the path of the transfer of electrons from NADH to coenzyme Q. In contrast, the transfer of electrons from FADH₂ to coenzyme Q is downstream of CII. Thus even a large Gibbs force ('decrease in free energy') in FADH₂→Q would fail to drive the coupled process of proton translocation through CII, since the Gibbs force in S→FADH₂ is missing. (In parentheses: None of these steps are coupled to ATP synthesis. Redox-driven proton translocation should not be confused with pmF-driven phosphorylation of ADP).

(3) 'Electrons from succinate enter the electron transport chain via FADH₂ in complex II. They are then transferred to coenzyme Q and carried through the rest of the electron transport chain ..'

Comment: The ambiguity - CII receives electrons (1) from succinate, yet it is suggested that electrons (from succinate) enter the electron transport chain (3) via FADH₂ in complex II. Then two contrasting definitions are implied of the term 'electron transport chain' or better membrane-bound electron transfer system, membrane-ETS. (a) If CII is part of the membrane-ETS, then electrons enter the membrane-ETS from succinate (1) but not from FADH₂. (b) If electrons enter the 'electron transport chain' via FADH₂ in complex II (3), then CII would be upstream and hence not part of the membrane-ETS (to which conclusion, obviously - see Figure - nobody would agree). Dismissing concept (b) of the membrane-ETS, then remains the ambiguity, if electrons enter the membrane-ETS from FADH₂ (3, wrong) or from succinate (1, correct).

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