Thomas 2011 Mitochondrion: Difference between revisions
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|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|injuries=RONS; Oxidative Stress | |injuries=RONS; Oxidative Stress | ||
|organism=Mouse | |organism=Human, Mouse | ||
|tissues=Cardiac Muscle, Skeletal Muscle, Neurons; Brain | |tissues=Cardiac Muscle, Skeletal Muscle, Neurons; Brain, Fibroblast | ||
|preparations=Homogenate, Isolated Mitochondria | |preparations=Homogenate, Isolated Mitochondria | ||
|topics=ATP; ADP; AMP; PCr | |topics=ATP; ADP; AMP; PCr | ||
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Revision as of 16:49, 26 March 2012
Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP Jr (2011) Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11: 108-118. |
ยป [[Has info::PMID:20727424 ]]
Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP (2011) Mitochondrion
Abstract: Recombinant human mitochondrial transcription factor A protein (rhTFAM) was evaluated for its acute effects on cultured cells and chronic effects in mice. Fibroblasts incubated with rhTFAM acutely increased respiration in a chloramphenicol-sensitive manner. SH-SY5Y cells showed rhTFAM concentration-dependent reduction of methylpyridinium (MPP+)-induced oxidative stress and increases in lowered ATP levels and viability. Mice treated with weekly i.v. rhTFAM showed increased mitochondrial gene copy number, Complex I protein levels and ATP production rates; oxidative damage to proteins was decreased ~50%. rhTFAM treatment improved motor recovery rate after treatment with MPTP and dose-dependently improved survival in the lipopolysaccharide model of endotoxin sepsis. โข Keywords: recombinant TFAM, mitochondrial biogenesis, ATP synthesis, MPTP, sepsis
โข O2k-Network Lab: US_VA Richmond_Bennett JP
Labels:
Stress:RONS; Oxidative Stress Organism: Human, Mouse Tissue;cell: Cardiac Muscle, Skeletal Muscle, Neurons; Brain, Fibroblast Preparation: Homogenate, Isolated Mitochondria
Regulation: ATP; ADP; AMP; PCr
HRR: Oxygraph-2k